PXD025899

PXD025899 is an original dataset announced via ProteomeXchange.

Title	Human eccrine sweat shotgun proteomics for personalized monitoring and therapy
Description	The potential of eccrine sweat as a bio-fluid of interest for diagnosis and personalized therapy has not yet been fully evaluated, due to the lack of in-depth sweat characterization studies. Thanks to recent developments in the field of omics together with the availability of accredited eccrine sweat collection methods, the analysis of human sweat may now be envisioned as a standardized, non-invasive test for individualized monitoring and personalized medicine. Here, we characterized individual sweat samples, collected from 28 healthy adult volunteers under the most standardized sampling methodology, by applying an optimized Shotgun proteomic analysis. This deep characterization of the sweat proteome allowed the identification of about 1000 unique proteins from which 347 were identified across all samples. Annotation-wise, the study of the sweat proteome unveiled the over-representation of newly addressed Actin dynamics, oxidative stress and proteasome-related functions, in addition to well-described proteolysis and anti-microbial immunity. The sweat proteome composition appeared to be correlated to the inter-individual variability of sweat secretion parameters (water and solute losses). Besides, both gender-exclusive proteins and gender-specific protein abundances were highlighted in spite of the high similarity between human female and male sweat proteomes. In conclusion, standardized sample collection coupled to optimized shotgun proteomics significantly improved the depth of sweat proteome coverage, far beyond previous similar studies. The identified proteins were involved in many diverse biological processes and molecular functions indicating the potential of this bio-fluid as a valuable biological matrix for further studies. Addressing sweat variability, our results prove the proteomic profiling of sweat to be a promising bio-fluid for individualized, non-invasive monitoring and personalized medicine.
HostingRepository	PRIDE
AnnounceDate	2021-11-03
AnnouncementXML	Submission_2021-11-03_06:13:23.538.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD025899
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Bastien Burat
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; deamidated residue; iodoacetamide derivatized residue
Instrument	Q Exactive Plus

Revision	Datetime	Status	ChangeLog Entry
0	2021-05-09 07:51:31	ID requested
⏵ 1	2021-11-03 06:13:24	announced

Burat B, Reynaerts A, Baiwir D, Fl, é, ron M, Eppe G, Leal T, Mazzucchelli G, Characterization of the Human Eccrine Sweat Proteome-A Focus on the Biological Variability of Individual Sweat Protein Profiles. Int J Mol Sci, 22(19):(2021) [pubmed]

submitter keyword: Human, Eccrine sweat, LC-MS/MS, Shotgun proteomics

Gauthier EPPE
contact affiliation	Laboratory of Mass Spectrometry-Director MolSys Research Unit-Director CART (Center for Analytical Research and Technology)-Director Liège Université, Liège, Belgium
contact email	G.Eppe@uliege.be
lab head
Bastien Burat
contact affiliation	MSLab, MolSys RU, University of Liege
contact email	bastien.burat@etu.unilim.fr
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/11/PXD025899

PRIDE project URI

• PRIDE
  1. PXD025899
     1. Label: PRIDE project
     2. Name: Human eccrine sweat shotgun proteomics for personalized monitoring and therapy