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PXD025891

PXD025891 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleComprehensive proteomics analyses of IL-15 driven activation of intestinal intraepithelial lymphocytes
DescriptionIntestinal intraepithelial lymphocytes (IEL) are an abundant population of tissue-resident T cells that protect the gut from pathogens and maintain intestinal homeostasis. The cytokine IL-15 is trans-presented by epithelial cells to IEL in complex with the IL-15 receptor α chain (IL-15Rα) and plays essential roles both in maintaining IEL homeostasis, and in inducing IEL activation in response to epithelial stress. When overproduced, IL-15 is a key driver of the gluten-induced enteropathy Coeliac disease, through cytotoxic activation of IEL. To better understand how IL-15 directly regulates both homeostatic and inflammatory functions of IEL, we performed quantitative proteomics of IL-15/Rα-stimulated murine IEL, sorted into their 3 main subpopulations, TCRγδ CD8αα, TCRαβ CD8αβ and TCRαβ CD8αα expressing IEL. The data reveal that high IL-15/Rα stimulation licenses cell cycle activation, upregulates the biosynthetic machinery in IEL, increases mitochondrial respiratory capacity and induces expression of cell surface immune receptors and adhesion proteins that potentially drive IEL activation.
HostingRepositoryPRIDE
AnnounceDate2021-05-10
AnnouncementXMLSubmission_2021-07-20_02:31:32.889.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterRenata Soares
SpeciesList scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationListNo PTMs are included in the dataset
InstrumentLTQ Orbitrap Velos
Dataset History
RevisionDatetimeStatusChangeLog Entry
02021-05-09 07:45:08ID requested
12021-05-10 03:48:45announced
22021-07-20 02:31:33announced2021-07-20: Updated publication reference for PubMed record(s): 34257288.
Publication List
James OJ, Vandereyken M, Marchingo JM, Singh F, Bray SE, Wilson J, Love AG, Swamy M, IL-15 and PIM kinases direct the metabolic programming of intestinal intraepithelial lymphocytes. Nat Commun, 12(1):4290(2021) [pubmed]
Keyword List
submitter keyword: Intraepithelial lymphocytes
Interleukin-15
quantitative proteomics
Coeliac disease
histone ruler
LFQ
Contact List
Mahima Swamy
contact affiliationMRC Protein Phosphorylation and Ubiquitylation Unit (PPU), School of Life Sciences, University of Dundee, Dundee, United Kingdom.
contact emailm.swamy@dundee.ac.uk
lab head
Renata Soares
contact affiliationMRC-PPU, University of Dundee
contact emailr.filipesoares@dundee.ac.uk
dataset submitter
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Dataset FTP location
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