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PXD025890 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleIdentification of RNF111 substrates by integrative ubiquitome and proteome analysis
DescriptionIn the present work, we sought to identify exhaustively the endogenous substrates ubiquitinated and degraded by the E3 ubiquitin ligase RNF111 in presence of TGF-β signaling by performing label free quantitative proteomics after enrichment of ubiquitinated proteins (ubiquitome) in parental U2OS cell line compared to U2OS CRISPR engineered clones expressing a truncated form of RNF111 devoid of C-terminal Ring domain. We compare two methods of enrichment for ubiquitinated proteins prior to mass spectrometry proteomics analysis, the diGly remnant peptide immunoprecipitation with a K-e-GG antibody and a novel approach using protein immunoprecipitation with an ubiquitin pan (Pan UB) nanobody that recognizes all ubiquitin chains and mono-ubiquitination. These ubiquitomes were compared to the corresponding proteome to identify proteins ubiquitinated and degraded by RNF111 upon TGF-β signaling pathway.
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterVictor Laigle
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListubiquitination signature dipeptidyl lysine
InstrumentOrbitrap Exploris 480; Q Exactive HF-X; Orbitrap Fusion
Dataset History
RevisionDatetimeStatusChangeLog Entry
02021-05-09 07:43:33ID requested
12021-11-17 09:14:49announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: RNF111, E3 ubiquitin ligase, ubiquitome, TGF-beta
Contact List
Damarys Loew
contact affiliationHead of the Curie Institute Mass Spectrometry Platform
contact emaildamarys.loew@curie.fr
lab head
Victor Laigle
contact affiliationMass Spectrometry Proteomics Facility (LSMP) - Institut Curie
contact emailvictor.laigle@curie.fr
dataset submitter
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