PXD025890 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Identification of RNF111 substrates by integrative ubiquitome and proteome analysis |
Description | In the present work, we sought to identify exhaustively the endogenous substrates ubiquitinated and degraded by the E3 ubiquitin ligase RNF111 in presence of TGF-β signaling by performing label free quantitative proteomics after enrichment of ubiquitinated proteins (ubiquitome) in parental U2OS cell line compared to U2OS CRISPR engineered clones expressing a truncated form of RNF111 devoid of C-terminal Ring domain. We compare two methods of enrichment for ubiquitinated proteins prior to mass spectrometry proteomics analysis, the diGly remnant peptide immunoprecipitation with a K-e-GG antibody and a novel approach using protein immunoprecipitation with an ubiquitin pan (Pan UB) nanobody that recognizes all ubiquitin chains and mono-ubiquitination. These ubiquitomes were compared to the corresponding proteome to identify proteins ubiquitinated and degraded by RNF111 upon TGF-β signaling pathway. |
HostingRepository | PRIDE |
AnnounceDate | 2021-11-17 |
AnnouncementXML | Submission_2021-11-17_09:14:49.094.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Victor Laigle |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | ubiquitination signature dipeptidyl lysine |
Instrument | Orbitrap Exploris 480; Q Exactive HF-X; Orbitrap Fusion |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2021-05-09 07:43:33 | ID requested | |
⏵ 1 | 2021-11-17 09:14:49 | announced | |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: RNF111, E3 ubiquitin ligase, ubiquitome, TGF-beta |
Contact List
Damarys Loew |
contact affiliation | Head of the Curie Institute Mass Spectrometry Platform |
contact email | damarys.loew@curie.fr |
lab head | |
Victor Laigle |
contact affiliation | Mass Spectrometry Proteomics Facility (LSMP) - Institut Curie |
contact email | victor.laigle@curie.fr |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD025890
- Label: PRIDE project
- Name: Identification of RNF111 substrates by integrative ubiquitome and proteome analysis