PXD025886

PXD025886 is an original dataset announced via ProteomeXchange.

Title	Site-specific N- and O-glycosylation Analysis of Human Plasma Fibronectin
Description	Human plasma fibronectin is an adhesive protein that plays a crucial role in wound healing. Many studies had indicated that glycans may mediate the expression and functions of fibronectin, yet a comprehensive understanding of its glycosylation is still missing. Here, we performed a comprehensive N- and O-glycosylation mapping of human plasma fibronectin, and quantified the occurrence of each glycoform in a site-specific manner. Intact N-glycopeptides were enriched by zwitterionic hydrophilic interaction chromatography, and N-glycosites sites were localized by the 18O-labeling method. O-glycopeptide enrichment and O-glycosite identification were achieved by an enzyme-assisted site-specific extraction method. An RP–LC–MS/MS system functionalized with Collision-Induced Dissociation and stepped normalized collision energy (sNCE)-HCD tandem mass was applied to analyze the glycoforms of fibronectin. A total of 6 N-glycosites and 53 O-glycosites were identified, which were occupied by 3842 N-glycoforms and 16 O-glycoforms, respectively. Furthermore, 81.4% of N-glycans were either fucosylated, sialylated, or with both modifications77.31% of N-glycans were sialylated, while O-glycosylation was dominated by the sialyl-T antigen. These site-specific glycosylation patterns on human fibronectin can facilitate functional analyses of fibronectin and therapeutics development.
HostingRepository	PRIDE
AnnounceDate	2022-02-17
AnnouncementXML	Submission_2022-02-17_03:12:20.757.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Ding Liu
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	(18)O label at both C-terminal oxygens; monohydroxylated residue; deamidated residue; iodoacetamide derivatized residue
Instrument	LTQ Orbitrap Elite

Revision	Datetime	Status	ChangeLog Entry
0	2021-05-07 09:46:36	ID requested
⏵ 1	2022-02-17 03:12:21	announced

Liu D, Wang S, Zhang J, Xiao W, Miao CH, Konkle BA, Wan XF, Li L, Site-Specific N- and O-Glycosylation Analysis of Human Plasma Fibronectin. Front Chem, 9():691217(2021) [pubmed]

submitter keyword: Fibronectin, glycosylation , OpeRator, stepped normalized collision energy, mass spectrometer

Lei Li
contact affiliation	1Department of Chemistry, Georgia State University, Atlanta, GA
contact email	lli22@gsu.edu
lab head
Ding Liu
contact affiliation	Georgia State University
contact email	dliu14@gsu.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD025886
     1. Label: PRIDE project
     2. Name: Site-specific N- and O-glycosylation Analysis of Human Plasma Fibronectin