PXD025883

PXD025883 is an original dataset announced via ProteomeXchange.

Title	LC-MS/MS analysis of NGF proteins purified from cerebrospinal fluid
Description	The aim of the project is to develop and validate a new method to measure proNGF in the cerebrospinal fluid of living patients, and to use it as a biomarker of Alzheimer’s disease (AD). This method, a novel proNGF immunoassay, is based on molecular size separation by capillary electrophoresis and is performed with Simple Wes: this technique produces different immunoreactive peaks whose area can be calculated to search for a quantitative relation with AD. Our purpose was to confirm and validate by mass spectrometry analysis the presence of NGF in the selected peaks
HostingRepository	PRIDE
AnnounceDate	2022-02-17
AnnouncementXML	Submission_2022-02-17_03:11:48.825.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD025883
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Serena Camerini
SpeciesList	scientific name: Rattus norvegicus (Rat); NCBI TaxID: 10116; scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	iodoacetamide derivatized residue
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2021-05-07 09:46:19	ID requested
⏵ 1	2022-02-17 03:11:49	announced

Malerba F, Arisi I, Florio R, Zecca C, Dell'Abate MT, Bruni Ercole B, Camerini S, Casella M, Logroscino G, Cattaneo A, proNGF Measurement in Cerebrospinal Fluid Samples of a Large Cohort of Living Patients With Alzheimer's Disease by a New Automated Immunoassay. Front Aging Neurosci, 13():741414(2021) [pubmed]

submitter keyword: NGF, Alzheimer, cerebrospinal fluid, mass spectrometry

Serena Camerini
contact affiliation	Istituto superiore di Sanità
contact email	Serena.camerini@iss.it
lab head
Serena Camerini
contact affiliation	Istituto superiore di sanità
contact email	serena.camerini@iss.it
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/02/PXD025883

PRIDE project URI

• PRIDE
  1. PXD025883
     1. Label: PRIDE project
     2. Name: LC-MS/MS analysis of NGF proteins purified from cerebrospinal fluid