<<< Full experiment listing


PXD025874 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleProteomic analysis of healthy and hyperglycemic murine fetal kidneys
DescriptionAlteration of kidney morphogenesis in diabetic pregnancies is poorly described, especially changes in the extracellular matrix (ECM) and glomerular basement membrane (GBM during glomerulogenesis. Addressing the ECM proteome, or matrisome, in the mouse fetal kidney in a healthy and diabetic environment will improve understanding about the association between ECM changes in the kidney as potential reprogramming mechanisms of kidney differentiation in diabetic pregnancies. Therefore, to better understand ECM composition and remodelling in kidney developmental and appreciate the alterations associated to the maternal diabetes, this project aimed to define the matrisome in the mouse fetal kidney on embryonic day 19. For this, we used an ECM enrichment approach combined with high resolution label-free tandem mass spectrometry to define the matrisome in the fetal mouse kidney (healthy and hyperglycemic) to test the hypothesis that maternal diabetes influences ECM composition, assembly and, therefore, biology.
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterLouise Hopkinson
SpeciesList scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationListmonohydroxylated residue; acetylated residue; iodoacetamide derivatized residue
InstrumentQ Exactive HF
Dataset History
RevisionDatetimeStatusChangeLog Entry
02021-05-07 04:26:22ID requested
12021-10-13 00:01:57announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: Kidney, extracellular matrix, mass spectrometry, enrichment, basement membrane, diabetes
Contact List
Rachel Lennon
contact affiliationWellcome Centre for Cell-matrix biology, University of Manchester, UK.
contact emailrachel.lennon@manchester.ac.uk
lab head
Louise Hopkinson
contact affiliationUniversity of Manchester
contact emaillouise.hopkinson@postgrad.manchester.ac.uk
dataset submitter
Full Dataset Link List
Dataset FTP location
PRIDE project URI
Repository Record List
[ + ]