PXD025854

PXD025854 is an original dataset announced via ProteomeXchange.

Title	Brown adipocytes proteome upon acute adrenergic stimulation
Description	Mice and other small mammals heavily rely on UCP1-mediated non-shivering thermogenesis in the cold, and thus the unexpected cold resistance of UCP1-knockout (UCP1KO) mice has always been and still is an unresolved mystery inextricably intertwined with alternative means of thermogenesis and futile substrate cycles. Several potentially thermogenic futile substrate cycles in adipose tissue of WT and UCP1KO mice have been reported in the literature. We hypothesized that isoproterenol treatment of brown adipocytes from UCP1KO mice would cause an immediate acceleration of futile substrate cycling, and entail a consecutive upregulation of the involved enzymes to recruit additional capacity. In this study, we analyzed changes in the proteome of wild type (WT) and UCP1KO adipocytes during acute adrenergic stimulation to detect characteristic molecular signatures, which allowed us to narrow down our search to potential candidates related to Ca2+ and lipid metabolism.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:52:23.112.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Piero Giansanti
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	phosphorylated residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2021-05-07 04:12:09	ID requested
1	2022-04-25 19:18:05	announced
2	2022-05-06 10:45:40	announced	2022-05-06: Updated publication reference for DOI(s): doi.org/10.1016/j.molmet.2022.101499.
⏵ 3	2023-11-14 08:52:23	announced	2023-11-14: Updated project metadata.

Dataset with its publication pending

submitter keyword: proteomics,Brown adipocyte, mass spectrometry, proteome

Bernhard Kuster
contact affiliation	Chair of Proteomics and Bioanalytics, TUM School of Life Sciences, Technical University of Munich, Freising, Germany
contact email	kuster@tum.de
lab head
Piero Giansanti
contact affiliation	Technical University of Munich
contact email	piero.giansanti@tum.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/04/PXD025854

PRIDE project URI

• PRIDE
  1. PXD025854
     1. Label: PRIDE project
     2. Name: Brown adipocytes proteome upon acute adrenergic stimulation