PXD025852

PXD025852 is an original dataset announced via ProteomeXchange.

Title	An in situ ascorbate peroxidase 2-based labeling method and its application in detecting exosome proteins released from mouse proximal tubule cells under oxidative stress
Description	Here, we developed an in situ labeling approach to identify exosome-loaded proteins, which bypasses the traditional exosome isolation steps. In this method, an engineered ascorbate peroxidase targeting to endosomes-destined exosomes was used to label proteins loaded inside exosomes released from kidney proximal tubule-derived cells. Mass spectrometry analysis of the kidney proximal tubule-derived cells identified oxidative stress-induced exosome proteome alteration.
HostingRepository	PRIDE
AnnounceDate	2022-08-12
AnnouncementXML	Submission_2022-08-12_02:14:58.553.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Sang-Ho Kwon
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	biotinylated residue
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2021-05-06 23:41:01	ID requested
⏵ 1	2022-08-12 02:14:59	announced

Lee BR, Lee TJ, Oh S, Li C, Song JA, Marshall B, Zhi W, Kwon SH, Ascorbate peroxidase-mediated in situ labelling of proteins in secreted exosomes. J Extracell Vesicles, 11(6):e12239(2022) [pubmed]

submitter keyword: Exosomes, APEX2, mouse, kidney, oxidative stress, LC-MS/MS

Sang-Ho Kwon
contact affiliation	Department of Cellular Biology and Anatomy, Medical College of Georgia, Augusta University, Augusta, GA, USA
contact email	KKWON@augusta.edu
lab head
Sang-Ho Kwon
contact affiliation	Department of Cellular Biology and Anatomy, Medical College of Georgia, Augusta University, Augusta, GA, USA
contact email	kkwon@augusta.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD025852
     1. Label: PRIDE project
     2. Name: An in situ ascorbate peroxidase 2-based labeling method and its application in detecting exosome proteins released from mouse proximal tubule cells under oxidative stress