PXD025847
PXD025847 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Comprehensive Analysis of the Glycome and Glycoproteome of Bovine Milk-Derived Exosomes |
| Description | Bovine milk-derived exosomes (BMDEs) have potential applications in the pharmaceutical industry as drug delivery carriers. A comprehensive analysis of protein glycosylation in exosomes is necessary to elucidate the process of target delivery. In this work, free oligosaccharides (FOSs), O-glycans and N-glycans in BMDEs and whey were first analyzed through multiple derivation strategies. In summary, 13 FOSs, 44 O-glycans and 94 N-glycans were identified in bovine milk. To analyze the site-specific glycosylation of glycoproteins, a one-step method was used to enrich and characterize intact glycopeptides. A total of 1359 proteins including 114 glycoproteins were identified and most located in exosomes. Approximately 95 glycopeptides were initially discovered and 5 predicted glycosites were confirmed in BMDEs. Collectively, these findings revealed the characterization and distribution of glycans and glycoproteins in BMDEs, providing insight into the potential applications of BMDEs in drug delivery and food science. |
| HostingRepository | iProX |
| AnnounceDate | 2021-05-07 |
| AnnouncementXML | Submission_2023-08-28_00:35:03.163.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Wenyan Chen |
| SpeciesList | scientific name: Bos taurus; NCBI TaxID: 9913; |
| ModificationList | No PTMs are included in the dataset |
| Instrument | Orbitrap Fusion |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2021-05-06 20:29:13 | ID requested | |
| 1 | 2021-05-06 20:30:35 | announced | |
| ⏵ 2 | 2023-08-28 00:35:05 | announced | 2023-08-28: Update publication information. |
Publication List
| Chen W, Wang R, Li D, Zuo C, Wen P, Liu H, Chen Y, Fujita M, Wu Z, Yang G, Comprehensive Analysis of the Glycome and Glycoproteome of Bovine Milk-Derived Exosomes. J Agric Food Chem, 68(45):12692-12701(2020) [pubmed] |
Keyword List
| submitter keyword: Bovine milk-derived exosomes (BMDEs)，Glycoproteome，Mass spectrometry，Intact glycopeptide |
Contact List
| Ganglong Yang | |
|---|---|
| contact affiliation | Jiangnan university |
| contact email | glyanglife@jiangnan.edu.cn |
| lab head | |
| Wenyan Chen | |
| contact affiliation | Jiangna university |
| contact email | 593969391@qq.com |
| dataset submitter | |
Full Dataset Link List
| iProX dataset URI |
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