PXD025845

PXD025845 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Acylated peptide enrichment utilizing lysine deacylases for lysine acylomics
Description	Reversible acylation of lysine ε-amino groups, e.g., acetylation, succinylation, maronylation, and myristoylation, is involved in basic physiological processes such as metabolism, cell signaling and aging. In this study, we developed a novel enrichment method for acylated peptides without the use of antibodies, in which endogenously acylated peptides are deacylated by recombinant lysine deacylases based on the enzyme-substrate relationship and enriched by N-hydroxysuccinimidyl chemistry for identification of the acylated sites by nanoscale liquid chromatography-tandem mass spectrometric analysis. To demonstrate the validity of this acylomics platform, we used it to identify acylated sites on chemically acylated model protein samples. We also applied it to the nuclei of HeLa cells to identify endogenous acylated sites.
HostingRepository	jPOST
AnnounceDate	2021-06-03
AnnouncementXML	Submission_2022-09-18_03:07:52.396.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Kazuya Tsumagari
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	S-carboxamidomethyl-L-cysteine; alpha-amino dimethylated residue; N6,N6-dimethyl-L-lysine; 2x(13)C,4x(2)H labeled dimethylated L-lysine; Dimethyl:2H(4)13C(2); L-methionine sulfoxide; CAMthiopropanoyl; unknown modification; unknown modification; unknown modification
Instrument	TripleTOF 5600; Orbitrap Fusion Lumos

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2021-05-06 17:39:57	ID requested
1	2021-06-03 07:05:53	announced
⏵ 2	2022-09-18 03:07:53	announced	2022-09-18: Updated FTP location.

Publication List

Tsumagari K, Ishihama Y, Acylated peptide enrichment utilizing lysine deacylases for lysine acylomics. Biochem Biophys Res Commun, 563():60-65(2021) [pubmed]

Tsumagari K, Ishihama Y, Acylated peptide enrichment utilizing lysine deacylases for lysine acylomics. Biochem Biophys Res Commun, 563():60-65(2021) [pubmed]

Keyword List

submitter keyword: lysine acetylation, acetylproteomics, acetylated peptide enrichment

submitter keyword: lysine acetylation, acetylproteomics, acetylated peptide enrichment

Contact List

Yasushi Ishihama
lab head
Kazuya Tsumagari
contact affiliation	Keio University
dataset submitter

Full Dataset Link List

jPOST dataset URI
Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.jpostdb.org/JPST000634/

jPOST dataset URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.jpostdb.org/JPST000634/