PXD025841

PXD025841 is an original dataset announced via ProteomeXchange.

Title	An infection-activated redox switch in the lysosomal protease legumain promotes tumor growth
Description	Oxidative stress is a hallmark of tumorigenic infections, yet the underlying oxidation events that contribute to tumor growth remain poorly understood. By using chemical proteomics to map cysteine reactivity in human gastric cells, we determined that infection with the cancer-causing bacterium Helicobacter pylori induces oxidation of the lysosomal protease legumain at Cys219. The site-specific loss of Cys219 reactivity dysregulates intracellular legumain processing and localization in H. pylori-infected cells and increases tumor growth and mortality in a xenograft model. These findings establish a link between cysteine oxidation and tumorigenic signaling during bacterial infection and underscore the importance of oxidative post-translational modifications in tumor growth.
HostingRepository	PRIDE
AnnounceDate	2022-03-21
AnnouncementXML	Submission_2022-03-21_15:46:32.558.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Daniel Bak
SpeciesList	scientific name: Helicobacter pylori J99 (Campylobacter pylori J99); NCBI TaxID: 85963; scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	iodoacetamide derivatized residue
Instrument	LTQ Orbitrap

Revision	Datetime	Status	ChangeLog Entry
0	2021-05-06 08:33:19	ID requested
⏵ 1	2022-03-21 15:46:32	announced

Dataset with its publication pending

submitter keyword: Helicobacter pylori, cysteine oxidation, redox-signaling

Eranthie Weerapana
contact affiliation	Chemistry Department, Boston College, USA
contact email	eranthie@bc.edu
lab head
Daniel Bak
contact affiliation	Boston College
contact email	bakd@bc.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/03/PXD025841

PRIDE project URI

• PRIDE
  1. PXD025841
     1. Label: PRIDE project
     2. Name: An infection-activated redox switch in the lysosomal protease legumain promotes tumor growth