PXD025840

PXD025840 is an original dataset announced via ProteomeXchange.

Title	Mapping interactome networks of FOSL1 and FOSL2 in human Th17 cells
Description	The differentiation of Th17 cells is controlled by a complex network of transcription factors (TFs), including FOS and JUN proteins of the AP-1 family. The FOS-like proteins, FOSL1 and FOSL2 have recently been reported to control Th17 responses. The molecular mechanisms dictating their roles, however, are unclear. Moreover, although the functions of AP-1 TFs are largely governed by their protein-protein interactions, these are also poorly characterized in this milieu. Using affinity purification in combination with mass-spectrometry we established the first interactomes of FOSL1 and FOSL2 in human Th17 cells. In addition to their known interactions with JUN proteins, our analysis identified several novel binding partners of FOSL factors. Gene ontology analysis revealed RNA binding was enriched as the major functionality for FOSL1 and FOSL2 associated proteins, thereby suggesting possible mechanistic links that have not been studied before. Intriguingly, 29 interactors were found to be shared between FOSL1 and FOSL2, which included crucial regulators of Th17-fate. These findings, including unique and shared interactions, were validated using parallel reaction monitoring targeted mass-spectrometry (PRM-MS), with additional measurements with other laboratory methods. Overall, this study provides key insights into interaction-based signalling mechanisms of FOSL1 and FOSL2, which potentially control Th17 cell-development and associated pathologies
HostingRepository	PanoramaPublic
AnnounceDate	2021-09-18
AnnouncementXML	Submission_2021-09-18_19:54:43.286.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Robert Moulder
SpeciesList	scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationList	Carbamidomethyl; Label:13C(6)15N(2); Label:13C(6)15N(4); Label:13C(6)15N(1)
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2021-05-06 08:15:53	ID requested
⏵ 1	2021-09-18 19:54:43	announced

Shetty A, Bhosale SD, Tripathi SK, Buchacher T, Biradar R, Rasool O, Moulder R, Galande S, Lahesmaa R. Interactome Networks of FOSL1 and FOSL2 in Human Th17 Cells. ACS Omega [Internet]. 2021 Sep 16

Available from: https://doi.org/10.1021/acsomega.1c03681

submitter keyword: Interactome, Th17, PRM, FOSL1, FOSL2

Riitta Lahesmaa
contact affiliation	Turku Bioscience, University of Turku and Åbo Akademi University
contact email	rilahes@utu.fi
lab head
Robert Moulder
contact affiliation	Turku Bioscience, University of Turku and Åbo Akademi University
contact email	robmou@utu.fi
dataset submitter

Panorama Public dataset URI