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PXD025838-1

PXD025838 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleProteomic analysis of induced pluripotent stem cell-derived kidney organoids
DescriptionKidney organoids were generated from a control iPSC line using a previously published protocol (https://doi.org/10.1038/nprot.2016.098). Organoids were collected at three timepoints during the protocol (day 14, 18 and 25) and prepared for proteomic analyses. The focus of the study was to define the proteomic composition of kidney organoids during differentiation with a particular emphasis on the extracellular matrix and its comparison to in vivo systems. Following a ample fractionation and matrix enrichment strategy, samples were prepared for high resolution label-free tandem mass spectrometry to define the proteomic composition of human kidney organoids.
HostingRepositoryPRIDE
AnnounceDate2021-10-13
AnnouncementXMLSubmission_2021-10-12_23:55:23.105.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterLouise Hopkinson
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListmonohydroxylated residue; acetylated residue; iodoacetamide derivatized residue
InstrumentQ Exactive HF
Dataset History
RevisionDatetimeStatusChangeLog Entry
02021-05-06 07:18:32ID requested
12021-10-12 23:55:23announced
Publication List
Dataset with its publication pending
Keyword List
ProteomeXchange project tag: Biology/Disease-Driven Human Proteome Project (B/D-HPP), Human Proteome Project
submitter keyword: Kidney, Kidney organoid, extracellular matrix, enrichment, mass spectrometry, basement membrane
Contact List
Rachel Lennon
contact affiliationWellcome Centre for Cell-matrix biology, University of Manchester, UK.
contact emailrachel.lennon@manchester.ac.uk
lab head
Louise Hopkinson
contact affiliationUniversity of Manchester
contact emaillouise.hopkinson@postgrad.manchester.ac.uk
dataset submitter
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Dataset FTP location
PRIDE project URI
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