PXD025834

PXD025834 is an original dataset announced via ProteomeXchange.

Title	Transcriptome and iTRAQ proteomic analysis reveal the molecular mechanism of anti- Candida albicans action of trichoderma acid, a potent novel drug candidate
Description	Candida albicans has become one of the main pathogens of fungal infection, which causes great threaten to human health. Hence, it is urgent to develop new anti- C. albicans drugs. A compound named as trichoderma acid was isolated from Trichoderma spirale , which showed stronger inhibitory effect towards C. albicans even than positive control nystatin. The transcriptome sequencing and iTRAQ-based sequencing of trichoderma acid-treated C. albicans were performed to investigate the molecular mechanism of anti- C. albicans. And the most significant differential genes were verified by qRT-PCR and Western blot analysis. The results indicated that the disruption of mitochondrial membrane potential, cell membrane, endoplasmic reticulum and the ribosomal in mitochondrial and cell wall were detected in trichoderma acidtreated C. albicans, resulting in the accumulation of ROS. The expression level of supereoxidase SOD was down-regulated, thus enhancing the concentration of ROS. High concentration of ROS led to the DNA damage and destruction of cell skeleton. And the expression levels of Rho-related GTP-binding protein RND3, the asparagine synthetase ASNS, peroxidases, glutathione S-transferase and heat shock protein HSP70 were significantly up-regulated in response to the apoptotic process and toxin stimulus, which was demonstrated by Western blot analysis. The transciptomic, proteomic and cellular analysis would provide anti- C. albicans mechanism of trichoderma acid and the defensive response mechanism of C. albicans.
HostingRepository	iProX
AnnounceDate	2021-05-06
AnnouncementXML	Submission_2023-04-03_00:39:35.104.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Wei Ye
SpeciesList	scientific name: Candida albicans; NCBI TaxID: 5476;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive Plus; Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2021-05-06 04:35:07	ID requested
⏵ 1	2023-04-03 00:39:35	announced

Ye W, Chen Y, Zhang W, Liu T, Liu Y, Li M, Li S, Xu L, Liu H, . Int J Mol Sci, 24(6):(2023) [pubmed]

submitter keyword: trichoderma acid, Candida albicans, iTRAQ, proteomic

Weimin Zhang
contact affiliation	Guangdong Institute of Microbiology
contact email	wmzhang@gdim.cn
lab head
Wei Ye
contact affiliation	Guangdong Institute of Microbiology
contact email	yewei0320@qq.com
dataset submitter

iProX dataset URI