PXD025830

PXD025830 is an original dataset announced via ProteomeXchange.

Title	Genetic Architecture of Protein Expression and its Regulation in the Mouse Brain
Description	Natural variation in protein expression is common in all organisms and contribute to phenotypic differences among individuals. While variation in gene expression at the transcript level has been extensively investigated, the genetic mechanisms underlying variation in protein expression have lagged considerably behind. Here we investigate genetic architecture of protein expression by profiling a deep mouse brain proteome of two inbred strains, C57BL/6J (B6) and DBA/2J (D2), and their reciprocal F1 hybrids using two-dimensional liquid chromatography coupled with tandem mass spectrometry (LC/LC-MS/MS) technology. By comparing protein expression levels in the four mouse strains, we observed 329 statistically significant differentially expressed proteins between the two parental strains and identified four common inheritance patterns, including dominant, additive, over- and under-dominant expression. We further applied the proteogenomic approach to detect variant peptides and define protein allele-specific expression (pASE).
HostingRepository	PRIDE
AnnounceDate	2021-11-01
AnnouncementXML	Submission_2021-11-01_04:25:31.662.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	xusheng wang
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	monohydroxylated residue
Instrument	Q Exactive HF; Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2021-05-06 01:34:42	ID requested
⏵ 1	2021-11-01 04:25:32	announced

Dataset with its publication pending

submitter keyword: Proteome, Mouse, Brain, Protein expression, Expression pattern, Protein regulation

Xusheng Wang
contact affiliation	Department of Biology, University of North Dakota, Grand Forks, ND 58202, USA.
contact email	xusheng.wang@und.edu
lab head
xusheng wang
contact affiliation	Proteomics
contact email	xushengwang78@gmail.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD025830
     1. Label: PRIDE project
     2. Name: Genetic Architecture of Protein Expression and its Regulation in the Mouse Brain