PXD025829

PXD025829 is an original dataset announced via ProteomeXchange.

Title	A Proteome-Wide Atlas of Lysine-Reactive Chemistry
Description	Recent advances in chemical proteomics have begun to characterize the reactivity and ligandability of lysines on a global scale. Yet, only a limited diversity of aminophilic electrophiles have been evaluated for interactions with the lysine proteome. Here, we report an in-depth profiling of >30 uncharted aminophilic chemotypes that greatly expands the content of ligandable lysines in human proteins. Aminophilic electrophiles showed disparate proteomic reactivities that range from selective interactions with a handful of lysines to, for a set of dicarboxaldehyde fragments, remarkably broad engagement of the covalent small molecule-lysine interactions captured by the entire library. We used these latter “scout� electrophiles to efficiently map ligandable lysines in primary human immune cells under stimulatory conditions. Finally, we show that aminophilic compounds perturb diverse biochemical functions through site-selective modification of lysines in proteins, including protein-RNA interactions implicated in innate immune responses. These findings support the broad potential of covalent chemistry for targeting functional lysines in the human proteome.
HostingRepository	PRIDE
AnnounceDate	2021-09-21
AnnouncementXML	Submission_2021-09-21_02:02:54.630.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Mikail Abbasov
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	iodoacetamide derivatized residue
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2021-05-06 01:34:41	ID requested
1	2021-09-20 10:12:07	announced
⏵ 2	2021-09-21 02:02:55	announced	2021-09-21: Updated project metadata.

Abbasov ME, Kavanagh ME, Ichu TA, Lazear MR, Tao Y, Crowley VM, Am Ende CW, Hacker SM, Ho J, Dix MM, Suciu R, Hayward MM, Kiessling LL, Cravatt BF, A proteome-wide atlas of lysine-reactive chemistry. Nat Chem, 13(11):1081-1092(2021) [pubmed]

10.1038/S41557-021-00765-4;

submitter keyword: Human immune cells, lysine reactive probes

Benjamin F. Cravatt
contact affiliation	Department of Chemistry, Scripps Research Institute
contact email	cravatt@scripps.edu
lab head
Mikail Abbasov
contact affiliation	Department of Chemistry and Chemical Biology Cornell University
contact email	mikail.abbasov@cornell.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/09/PXD025829

PRIDE project URI

• PRIDE
  1. PXD025829
     1. Label: PRIDE project
     2. Name: A Proteome-Wide Atlas of Lysine-Reactive Chemistry