PXD025827

PXD025827 is an original dataset announced via ProteomeXchange.

Title	Label-free quantitative proteomics identifies unique proteomes of clinical isolates of the Liverpool Epidemic Strain of Pseudomonas aeruginosa and laboratory strain PAO1
Description	Pseudomonas aeruginosa is a predominant pathogen in chronic lung infections in individuals with cystic fibrosis (CF). Epidemic strains of P. aeruginosa, such as the Liverpool Epidemic Strain (LES), are capable of transferring between CF patients and have been associated with increased hospital visits and antibiotic treatments. Comparative genomics and phenotypic assays have shown that antibiotic resistance profiles differ among LES isolates and that genotype–phenotype associations are difficult to establish for resistance phenotypes in clinical isolates of P. aeruginosa based on these comparisons alone. We compared two LES isolates, LESlike1 and LESB58, and the common laboratory strain P. aeruginosa PAO1 using label-free quantitative proteomics to more accurately predict functional differences between strains. The proteomes of the LES isolates were found to be more similar to each other than to PAO1. However, we also observed a number of differences in the abundance of proteins involved in quorum sensing, virulence, and antibiotic resistance, including in the comparison of LESlike1 and LESB58. Specifically, the proteomic data revealed a higher abundance of proteins involved in polymyxin and aminoglycoside resistance in LESlike1. Minimum inhibitory concentration assays confirmed that LESlike1 has higher resistance to antibiotics from these classes. These findings provide an example of the ability of proteomic data to complement genotypic and phenotypic studies to understand resistance in clinical isolates.
HostingRepository	PRIDE
AnnounceDate	2022-05-09
AnnouncementXML	Submission_2022-05-09_12:20:00.023.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Cezar Khursigara
SpeciesList	scientific name: Pseudomonas aeruginosa PAO1; NCBI TaxID: 208964; scientific name: Pseudomonas aeruginosa LESB58; NCBI TaxID: 557722; scientific name: Pseudomonas aeruginosa LESlike1; NCBI TaxID: 1408274;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2021-05-06 01:34:27	ID requested
⏵ 1	2022-05-09 12:20:00	announced

Goodyear MC, Garnier N, Krieger JR, Geddes-McAlister J, Khursigara CM, Label-free quantitative proteomics identifies unique proteomes of clinical isolates of the Liverpool Epidemic Strain of Pseudomonas aeruginosa and laboratory strain PAO1. Proteomics Clin Appl, 15(6):e2100062(2021) [pubmed]

submitter keyword: Pseudomonas aeruginosa, Liverpool Epidemic Strain, label-free quantitative proteomics, antibiotic resistance

Cezar M. Khursigara
contact affiliation	Department of Molecular and Cellular Biology, University of Guelph
contact email	ckhursig@uoguelph.ca
lab head
Cezar Khursigara
contact affiliation	University of Guelph
contact email	ckhursig@uoguelph.ca
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD025827
     1. Label: PRIDE project
     2. Name: Label-free quantitative proteomics identifies unique proteomes of clinical isolates of the Liverpool Epidemic Strain of Pseudomonas aeruginosa and laboratory strain PAO1