PXD025826 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | On-tissue spatially-resolved glycoproteomics guided by N-glycan imaging reveal global dysregulation of canine glioma glycoproteomic landscape |
Description | Here we present an approach to identify N-linked glycoproteins and deduce their spatial localization using a combination of MALDI N-glycan MSI and spatially-resolved glycoproteomics. We subjected glioma biopsies to on-tissue PNGaseF digestion and MALDI-MSI and found that the glycan HexNAc4-Hex5-NeuAc2 was predominantly expressed in necrotic regions of high-grade canine gliomas. To determine the underlying sialo-glycoprotein, various regions in adjacent tissue sections were subjected to microdigestion and manual glycoproteomic analysis. Results identified haptoglobin as the protein associated with HexNAc4-Hex5-NeuAc2, making our study the first report that directly links glycan imaging with intact glycopeptide identification. In total, our spatially-resolved glycoproteomics technique identified over 400 N-, O-, and S- glycopeptides from over 30 proteins, demonstrating the diverse array of glycosylation present on the tissue slides and the sensitivity of our technique. Ultimately, this proof-of-principle work demonstrates that spatially-resolved glycoproteomics greatly complement MALDI-MSI in understanding dysregulated glycosylation. |
HostingRepository | PRIDE |
AnnounceDate | 2023-11-14 |
AnnouncementXML | Submission_2023-11-14_08:44:07.425.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Soulaimane Aboulouard |
SpeciesList | scientific name: Canis lupus; NCBI TaxID: 9612; |
ModificationList | No PTMs are included in the dataset |
Instrument | Bruker Daltonics flex series |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2021-05-06 01:34:25 | ID requested | |
1 | 2023-03-11 14:06:21 | announced | |
⏵ 2 | 2023-11-14 08:44:08 | announced | 2023-11-14: Updated project metadata. |
Publication List
Malaker SA, Quanico J, Raffo-Romero A, Kobeissy F, Aboulouard S, Tierny D, Bertozzi CR, Fournier I, Salzet M, On-tissue spatially resolved glycoproteomics guided by N-glycan imaging reveal global dysregulation of canine glioma glycoproteomic landscape. Cell Chem Biol, 29(1):30-42.e4(2022) [pubmed] |
Keyword List
submitter keyword: glioblastoma, spatially-resolved, glycoproteomics, haptoglobin, sialic-acid, MALDI-MSI, hypersialylation,Glycan-imaging |
Contact List
Michel Salzet |
contact affiliation | Laboratoire PRISM INSERM U1192 Université de Lille Villeneuve d'ascq France |
contact email | michel.salzet@univ-lille.fr |
lab head | |
Soulaimane Aboulouard |
contact affiliation | PRISM |
contact email | soulaimane.aboulouard@univ-lille.fr |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD025826
- Label: PRIDE project
- Name: On-tissue spatially-resolved glycoproteomics guided by N-glycan imaging reveal global dysregulation of canine glioma glycoproteomic landscape