PXD025820

PXD025820 is an original dataset announced via ProteomeXchange.

Title	The molecular basis of regulation of bacterial capsule assembly by Wzc
Description	Bacterial extracellular polysaccharides (EPSs) play critical roles in virulence as well as representing valuable bioproducts. Many bacteria use a “Wzx-Wzy-dependent” mechanism to assemble EPSs, in a tightly controlled multi-protein process that couples glycan polymerisation at the inner membrane to translocation across the periplasm and outer membrane (OM). The tyrosine autokinase, Wzc, is required for both polymerization and translocation. The cryo-EM structure of dephosphorylated Wzc from E. coli reveals an octameric structure, where transmembrane helices create a large central cavity and connect the cytoplasmic tyrosine kinase domain to a periplasmic region containing helical bundles. Progressive autophosphorylation of Wzc’s tyrosine-rich C-terminus disassembles the octamer into a monomer and the cycling between these states in the cell drives function. The helical bundles are essential for function and their conformation responds to phosphorylation; most likely gating the OM translocase. We propose a molecular model for the regulation of EPS synthesis and transport by Wzc.
HostingRepository	PRIDE
AnnounceDate	2021-07-06
AnnouncementXML	Submission_2021-08-26_04:33:53.491.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Jani Bolla
SpeciesList	scientific name: Escherichia coli; NCBI TaxID: 562;
ModificationList	phosphorylated residue
Instrument	Orbitrap Eclipse

Revision	Datetime	Status	ChangeLog Entry
0	2021-05-05 22:45:18	ID requested
1	2021-07-05 21:31:57	announced
⏵ 2	2021-08-26 04:33:54	announced	2021-08-26: Updated publication reference for PubMed record(s): 34272394.

Yang Y, Liu J, Clarke BR, Seidel L, Bolla JR, Ward PN, Zhang P, Robinson CV, Whitfield C, Naismith JH, The molecular basis of regulation of bacterial capsule assembly by Wzc. Nat Commun, 12(1):4349(2021) [pubmed]

submitter keyword: E. coli, Phosphoproteomics, Tyrosine autokinase

Carol V Robinson
contact affiliation	Department of Chemistry, University of Oxford
contact email	carol.robinson@chem.ox.ac.uk
lab head
Jani Bolla
contact affiliation	University of Oxford, Oxford
contact email	jani.bolla@chem.ox.ac.uk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/07/PXD025820

PRIDE project URI

• PRIDE
  1. PXD025820
     1. Label: PRIDE project
     2. Name: The molecular basis of regulation of bacterial capsule assembly by Wzc