PXD025803

PXD025803 is an original dataset announced via ProteomeXchange.

Title	Human cytomegalovirus-induced host protein citrullination is crucial for viral replication
Description	Citrullination is the conversion of arginine-to-citrulline by protein arginine deiminases (PADs), whose dysregulation is implicated in the pathogenesis of various types of cancers and autoimmune diseases. Consistent with the ability of human cytomegalovirus (HCMV) to induce post-translational modifications of cellular proteins to gain a survival advantage, we show that HCMV infection of primary human fibroblasts triggers PAD-mediated citrullination of several host proteins, and that this activity promotes viral fitness. Citrullinome analysis reveals significant changes in deimination levels of both cellular and viral proteins, with interferon (IFN)-inducible protein IFIT1 being the most heavily deiminated one. As genetic depletion of IFIT1 strongly enhances HCMV growth, and in vitro IFIT1 citrullination impairs its ability to bind to 5’-pppRNA, we propose that viral-induced IFIT1 citrullination is a novel mechanism of HCMV evasion from host antiviral resistance. Overall, our findings point to a crucial role of citrullination in subverting cellular responses to viral infection.
HostingRepository	PRIDE
AnnounceDate	2021-07-20
AnnouncementXML	Submission_2021-07-20_00:59:53.012.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Virginie Girault
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	L-methionine sulfoxide; N-acylated residue; L-citrulline; iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2021-05-05 05:40:07	ID requested
⏵ 1	2021-07-20 00:59:53	announced

Griffante G, Gugliesi F, Pasquero S, Dell'Oste V, Biolatti M, Salinger AJ, Mondal S, Thompson PR, Weerapana E, Lebbink RJ, Soppe JA, Stamminger T, Girault V, Pichlmair A, Oroszl, á, n G, Coen DM, De Andrea M, Landolfo S, Human cytomegalovirus-induced host protein citrullination is crucial for viral replication. Nat Commun, 12(1):3910(2021) [pubmed]

submitter keyword: HCMV, IFIT1, citrullination

Andreas Pichlmair
contact affiliation	Institute of Virology, Technical University of Munich, Munich 81675, Germany
contact email	andreas.pichlmair@tum.de
lab head
Virginie Girault
contact affiliation	Institute of Virology - Technical University Munich
contact email	v.girault@tum.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/07/PXD025803

PRIDE project URI

• PRIDE
  1. PXD025803
     1. Label: PRIDE project
     2. Name: Human cytomegalovirus-induced host protein citrullination is crucial for viral replication