PXD025802

PXD025802 is an original dataset announced via ProteomeXchange.

Title	Trypanosoma brucei PH1-BioID proxisome
Description	Kinesins are motor proteins found in all eukaryotic lineages that move along microtubule tracks to mediate several cellular processes such as mitosis and intracellular transport of cargo. In trypanosomatids, the kinesin protein superfamily has undergone a prominent expansion, giving these protists one of the most diverse kinesin repertoires in eukaryotes. This has led to the emergence of two trypanosomatid-restricted groups of kinesins. Here, we characterize in Trypanosoma brucei TbKifX2, a hitherto orphaned kinesin that belongs to one of these groups. TbKifX2 tightly interacts with TbPH1, a kinesin-like protein named after a pleckstrin homology (PH) domain present within its carboxy terminal tail. TbKifX2 recruits TbPH1 to the microtubule quartet (MtQ), a characteristic cytoskeletal structure that runs adjacent to the flagellar attachment zone filament from the basal body to the anterior of the cell. The proximal proteome of TbPH1 has revealed four proteins that localize to structures found adjacent to the length of the MtQ, further confirming that the likely TbKifX1/TbPH1 heterodimer binds the MtQ along its whole length. Simultaneous ablation of both TbKifX2 and TbPH1 leads to the formation of prominent extrusions from the cell posterior. Thus, we have attributed a morphogenesis role to these two trypanosomatid-restricted proteins, and the unique cytoskeletal structure represented by the MtQ. We hypothesize that the putative TbKiX2/TbPH1 heterodimer may transport a cytokinesis auxiliary factor(s) along the MtQ to or from the T. brucei posterior. The cohort of proteins found in proximity to TbPH1 may represent one of these factors directly or be involved in their trafficking during cell division in trypanosomatids.
HostingRepository	PRIDE
AnnounceDate	2022-06-30
AnnouncementXML	Submission_2022-06-30_01:20:12.886.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Corinna Benz
SpeciesList	scientific name: Trypanosoma brucei; NCBI TaxID: 5691;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2021-05-05 05:40:06	ID requested
⏵ 1	2022-06-30 01:20:14	announced

Benz C, M, ü, ller N, Kaltenbrunner S, V, á, chov, á H, Vancov, á M, Luke, š J, Varga V, Hashimi H, Kinetoplastid-specific X2-family kinesins interact with a kinesin-like pleckstrin homology domain protein that localizes to the trypanosomal microtubule quartet. Mol Microbiol, 118(3):155-174(2022) [pubmed]

submitter keyword: Trypanosoma, BioID, kinesin-like protein

Julius Lukes
contact affiliation	Institute of Parasitology Biology Center, Czech Academy of Sciences Branišovská 31 370 05 České Budějovice CZECH REPUBLIC
contact email	jula@paru.cas.cz
lab head
Corinna Benz
contact affiliation	Institute of Parasitology Biology Center, Czech Academy of Sciences Branišovská 31 370 05 České Budějovice
contact email	corinna.benz@paru.cas.cz
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/06/PXD025802

PRIDE project URI

• PRIDE
  1. PXD025802
     1. Label: PRIDE project
     2. Name: Trypanosoma brucei PH1-BioID proxisome