PXD025785

PXD025785 is an original dataset announced via ProteomeXchange.

Title	Sequence analysis of Giardia virus proteins by LC-MS/MS
Description	Giardiasis, caused by the protozoan parasite Giardia duodenalis, is an intestinal diarrheal disease affecting almost one billion people worldwide. A small endosymbiotic dsRNA viruses, G. lamblia virus (GLV), genus Giardiavirus, family Totiviridae, might inhabit human and animal isolates of G. duodenalis. Three GLV genomes have been sequenced so far, and only one was intensively studied, moreover a positive correlation between GLV and parasite virulence is yet to be proved. To understand the biological significance of GLV infection in Giardia, the characterization of several GLV strains from naturally infected G. duodenalis isolates is necessary. In the contest of high-throughput sequencing of four GLVs strains, from Giardia isolates of human and animal origin, we report on a new, unclassified viral sequence (designed GdRV-2), unrelated to Giardiavirus, encoding and expressing for a single large protein with a RdRp domain homologous to Totiviridae and Botybirnaviridae. We have analyzed the sequence of the GLV capsid protein (CP) and RNA-dependent RNA polymerase (RdRp) by LC-MS/MS analysis using different enzymatic strategies.
HostingRepository	PRIDE
AnnounceDate	2021-07-05
AnnouncementXML	Submission_2021-07-05_03:32:05.593.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Serena Camerini
SpeciesList	scientific name: Giardia intestinalis; NCBI TaxID: 5741;
ModificationList	iodoacetamide derivatized residue
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2021-05-04 01:42:51	ID requested
⏵ 1	2021-07-05 03:32:06	announced

Marucci G, Zullino I, Bertuccini L, Camerini S, Cecchetti S, Pietrantoni A, Casella M, Vatta P, Greenwood AD, Fiorillo A, Lalle M, Isolates. Biomedicines, 9(6):(2021) [pubmed]

submitter keyword: Giardia duodenalis, Giardiavirus, proteomics

Serena Camerini
contact affiliation	istituto superiore di sanità
contact email	serena.camerini@iss.it
lab head
Serena Camerini
contact affiliation	Istituto superiore di sanità
contact email	serena.camerini@iss.it
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD025785
     1. Label: PRIDE project
     2. Name: Sequence analysis of Giardia virus proteins by LC-MS/MS