PXD025783

PXD025783 is an original dataset announced via ProteomeXchange.

Title	Mycobacterium smegmatis cultured in different carbon sources to induce in vitro cholesterol consumption reveals alterations in immune response after infection: a macrophage proteomics analysis.
Description	During Tuberculosis, Mycobacterium uses host macrophage cholesterol as a carbon and energy source. To mimic these conditions Mycobacterium smegmatis cultured in minimal medium (MM) has been used to induce cholesterol consumption in vitro. During cultivation, M. smegmatis con-sumes MM cholesterol and changes the accumulation of the cell wall compounds such as PIMs, LM and LAM, which plays an important role in its pathogenicity. These changes led to cell sur-face hydrophobicity modifications and H2O2 susceptibility. Furthermore, when M. smegmatis in-fects J774A.1 macrophages it induces granuloma-like structures formation. The present study aims to access macrophage molecular disturbances caused by M. smegmatis after cholesterol con-sumption through proteomics analyses.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_05:32:37.151.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Juliana Cassoli
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	iodoacetamide derivatized residue
Instrument	Synapt MS

Revision	Datetime	Status	ChangeLog Entry
0	2021-05-04 01:42:35	ID requested
1	2022-02-17 03:10:58	announced
⏵ 2	2024-10-22 05:32:43	announced	2024-10-22: Updated project metadata.

de Lima JB, da Silva Fonseca LP, Xavier LP, de Matos Macchi B, Cassoli JS, da Silva EO, da Silva Valadares RB, do Nascimento JLM, Santos AV, de Sena CBC, in Different Carbon Sources to Induce In Vitro Cholesterol Consumption Leads to Alterations in the Host Cells after Infection: A Macrophage Proteomics Analysis. Pathogens, 10(6):(2021) [pubmed]

10.3390/pathogens10060662;

submitter keyword: Mycobacterium

proteomics

cholesterol

infection

macrophages.

Juliana Silva Cassoli
contact affiliation	Institute of Biological Sciences, Federal University of Pará, Belém/PA, Brazil.
contact email	jscassoli@gmail.com
lab head
Juliana Cassoli
contact affiliation	University of Campinas
contact email	jscassoli@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/02/PXD025783

PRIDE project URI

• PRIDE
  1. PXD025783
     1. Label: PRIDE project
     2. Name: Mycobacterium smegmatis cultured in different carbon sources to induce in vitro cholesterol consumption reveals alterations in immune response after infection: a macrophage proteomics analysis.