PXD025770

PXD025770 is an original dataset announced via ProteomeXchange.

Title	Smooth muscle-specific MMP17 (MT4-MMP) defines the intestinal ECM niche
Description	Smooth muscle is an essential component of the intestine, both to maintain its structure and produce peristaltic and segmentation movements. However, very little is known about other putative roles that smooth muscle may have. Here, we show that smooth muscle is the dominant supplier of BMP antagonists, which are niche factors that are essential for intestinal stem cell maintenance. Furthermore, muscle-derived factors can render epithelium reparative and fetal-like, which includes heightened YAP activity. Mechanistically, we find that the matrix metalloproteinase MMP17, which is exclusively expressed by smooth muscle, is required for intestinal epithelial repair after inflammation- or irradiation-induced injury. Furthermore, we provide evidence that MMP17 affects intestinal epithelial reprogramming indirectly by cleaving the matricellular protein PERIOSTIN, which itself is able to activate YAP. Together, we identify an important signaling axis that firmly establishes a role for smooth muscle as a modulator of intestinal epithelial regeneration and the intestinal stem cell niche.
HostingRepository	PRIDE
AnnounceDate	2023-04-11
AnnouncementXML	Submission_2023-04-11_06:05:42.186.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	MirjamDamen
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2021-05-03 22:57:41	ID requested
1	2021-11-02 05:36:47	announced
⏵ 2	2023-04-11 06:05:43	announced	2023-04-11: Updated project metadata.

Mart, í, n-Alonso M, Iqbal S, Vornewald PM, Lindholm HT, Damen MJ, Mart, í, nez F, Hoel S, D, í, ez-S, á, nchez A, Altelaar M, Katajisto P, Arroyo AG, Oudhoff MJ, Smooth muscle-specific MMP17 (MT4-MMP) regulates the intestinal stem cell niche and regeneration after damage. Nat Commun, 12(1):6741(2021) [pubmed]

submitter keyword: smooth muscle, MMP17

MennoOudhoff
contact affiliation	Centre of Molecular Inflammation Research (CEMIR, www.ntnu.edu/cemir) Dept. of Clinical and Molecular Medicine Norwegian University of Science and Technology (NTNU) Trondheim
contact email	menno.oudhoff@ntnu.no
lab head
MirjamDamen
contact affiliation	Utrecht Univeristy
contact email	j.m.a.damen@uu.nl
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/11/PXD025770

PRIDE project URI

• PRIDE
  1. PXD025770
     1. Label: PRIDE project
     2. Name: Smooth muscle-specific MMP17 (MT4-MMP) defines the intestinal ECM niche