PXD025769

PXD025769 is an original dataset announced via ProteomeXchange.

Title	Jejunal mucosa proteomics unravel metabolic adaptive processes to mild chronic heat stress in dairy cows
Description	Climate change affects the duration and intensity of heat waves during summer months and jeopardizes animal health and welfare. High ambient temperatures cause heat stress in dairy cows resulting in a reduction of milk yield, feed intake, and alterations in gut barrier function. The objectives of this study were to investigate the mucosal amino acid, glucose and lactate metabolism, as well as the proteomic response of the small intestine in heat stressed (HS) Holstein dairy cows. Cows of the HS group (n = 5) were exposed for 4 days to 28°C (THI = 76) in a climate chamber. Percentage decrease in daily ad libitum intake of HS cows was calculated to provide isocaloric energy intake to pair-fed control cows kept at 15°C (THI = 60) for 4 days. The metabolite, mRNA and proteomic analyses revealed that HS induced incorrect protein folding, cellular destabilization, increased proteolytic degradation and protein kinase inhibitor activity, reduced glycolysis, and activation of NF-κB signaling, uronate cycling, pentose phosphate pathway, fatty acid and amino acid catabolism, mitochondrial respiration, ATPase activity and the antioxidative defence system. Our results highlight adaptive metabolic and immune mechanisms attempting to maintain the biological function in the small intestine of heat-stressed dairy cows.
HostingRepository	PRIDE
AnnounceDate	2022-02-17
AnnouncementXML	Submission_2022-02-17_03:03:50.889.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD025769
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Dirk Albrecht
SpeciesList	scientific name: Bos taurus (Bovine); NCBI TaxID: 9913;
ModificationList	monohydroxylated residue
Instrument	LTQ FT

Revision	Datetime	Status	ChangeLog Entry
0	2021-05-03 22:56:51	ID requested
⏵ 1	2022-02-17 03:03:51	announced

Koch F, Albrecht D, G, ö, rs S, Kuhla B, Jejunal mucosa proteomics unravel metabolic adaptive processes to mild chronic heat stress in dairy cows. Sci Rep, 11(1):12484(2021) [pubmed]

submitter keyword: Cow, Intestinal mucosa, LC-MSMS

Dirk Albrecht
contact affiliation	University of Greifswald Institute of Microbiology
contact email	dirkalbr@uni-greifswald.de
lab head
Dirk Albrecht
contact affiliation	EMAU
contact email	dirkalbr@uni-greifswald.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD025769
     1. Label: PRIDE project
     2. Name: Jejunal mucosa proteomics unravel metabolic adaptive processes to mild chronic heat stress in dairy cows