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PXD025767

PXD025767 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleA quantitative map of human primary microRNA processing sites
DescriptionMaturation of canonical microRNA (miRNA) is initiated by DROSHA that cleaves the primary transcript (pri-miRNA). Over 1,800 miRNA loci are annotated in humans, but it remains largely unknown if and at which sites the pri-miRNAs are cleaved by DROSHA. Here we performed in vitro processing on a full set of human pri-miRNAs (miRBase v21) followed by sequencing. This comprehensive profiling enabled us to classify miRNAs based on DROSHA-dependence and map their cleavage sites with respective processing efficiency measures. Only 758 pri-miRNAs are confidently processed by DROSHA, while the majority may be non-canonical or false entries. Analyses of the DROSHA-dependent pri-miRNAs show key cis-elements for processing. We observe widespread alternative processing as well as unproductive cleavage events such as “nick” or “inverse” processing. SRSF3 is a broad-acting auxiliary factor modulating alternative processing and suppressing unproductive processing. The profiling data and methods developed in this study will allow systematic analyses of miRNA regulation.
HostingRepositoryPRIDE
AnnounceDate2021-07-28
AnnouncementXMLSubmission_2021-07-27_21:57:47.658.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterKijun Kim
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListiodoacetamide derivatized residue
InstrumentOrbitrap Fusion Lumos
Dataset History
RevisionDatetimeStatusChangeLog Entry
02021-05-03 22:54:51ID requested
12021-07-27 21:57:48announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: MicroRNA
Pri-miRNA processing
DROSHA
DGCR8
Microprocessor
SRSF3
RNA pull-down assay
Contact List
V. Narry Kim
contact affiliationSeoul National University
contact emailnarrykim@snu.ac.kr
lab head
Kijun Kim
contact affiliationSeoul National University
contact emailwonderful@snu.ac.kr
dataset submitter
Full Dataset Link List
Dataset FTP location
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