PXD025765

PXD025765 is an original dataset announced via ProteomeXchange.

Title	HDX-MS Analysis of RORg:RORE:SRC3 Complexes
Description	The retinoic acid receptor-related orphan receptor γ (RORγ) is a ligand-dependent transcription factor that both underpins metabolic and immune functions and provides vantage to manipulate those processes pharmacologically. Despite its importance, our understanding of the ligand-dependent activities of RORγ is far from complete and developing a detailed structural model for RORγ pharmacology could provide a path towards the development of safe and efficacious therapeutics targeting the receptor. Herein, we examine the ligand-dependent assembly of recombinant RORγ:coregulator complexes on cognate DNA response elements using structural proteomics and small angle x-ray scattering. These studies reveal that the RORγ DNA binding domain can bind multiple different sequences of DNA, and that coregulatory proteins may be able to ‘sense’ the ligand- and DNA-bound status of RORγ. Overall, the efforts described herein will illuminate important aspects of RORγ activity and drug development that could lead to more efficacious treatments targeting this important receptor.
HostingRepository	PRIDE
AnnounceDate	2021-11-03
AnnouncementXML	Submission_2021-11-03_04:43:14.092.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Timothy Strutzenberg
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2021-05-03 22:53:16	ID requested
⏵ 1	2021-11-03 04:43:14	announced

Strutzenberg TS, Zhu Y, Novick SJ, Garcia-Ordonez RD, Doebelin C, He Y, Chang MR, Kamenecka TM, Edwards DP, Griffin PR, During Response Element Recognition and Coregulator Engagement. J Mol Biol, 433(22):167258(2021) [pubmed]

submitter keyword: RORg,SRC3,RORg Response Elements,Hydrogen Deuterium Exchange Mass Spectrometry,Protein-Protein Interaction,Protein-DNA Interaction

Patrick R. Griffin
contact affiliation	Department of Molecular Medicine The Scripps Research Institute
contact email	pgriffin@scripps.edu
lab head
Timothy Strutzenberg
contact affiliation	TSRI
contact email	tstrutze@scripps.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/11/PXD025765

PRIDE project URI

• PRIDE
  1. PXD025765
     1. Label: PRIDE project
     2. Name: HDX-MS Analysis of RORg:RORE:SRC3 Complexes