PXD025764

PXD025764 is an original dataset announced via ProteomeXchange.

Title	The impact of Zika virus exposure on the placental proteomic profile
Description	Zika virus (ZIKV) infection has caused severe unexpected clinical outcomes in neonates and adults during the recent outbreak in Latin America, particularly in Brazil. Congenital malformations associated with ZIKV have been frequently reported; nevertheless, the mechanism of vertical transmission and the involvement of placental cells remains unclear. In this study, we applied quantitative proteomics analysis in a floating explant model of chorionic villi of human placental tissues incubated with ZIKV and with ZIKV pre-adsorbed with anti-ZIKV envelope protein. The regulation of specific proteins was measured using immunofluorescence and immunoperoxidase assays. Altered levels of proteins were involved in cell proliferation, apoptosis, inflammatory processes, and the integrin-cytoskeleton complex. Antibody-opsonized ZIKV particles differentially modulated the pattern of protein expression in placental cells; this phenomenon may play a pivotal role in determining the course of infection and the role of mixed infections. These data fill gaps in our understanding of ZIKV in the placenta and help identify infection control targets.
HostingRepository	PRIDE
AnnounceDate	2021-12-30
AnnouncementXML	Submission_2021-12-30_14:20:38.779.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Giuseppe Palmisano
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2021-05-03 22:52:27	ID requested
⏵ 1	2021-12-30 14:20:39	announced

Rosa-Fernandes L, Bandeira C, Pour SZ, Benedetti VF, Ferreira D, Lorenzon AR, Moreli JB, Marinho CRF, Larsen MR, Zanotto P, Palmisano G, Bevilacqua E, The impact of Zika virus exposure on the placental proteomic profile. Biochim Biophys Acta Mol Basis Dis, 1868(1):166270(2022) [pubmed]

submitter keyword: Zika virus, proteomics, antibody opsonization, placenta, cell death

Estela M. A. F. Bevilacqua
contact affiliation	Institute of Biomedical Sciences, Department of Cell and Developmental Biology, University of São Paulo, 05508-000 São Paulo, SP, Brazil.
contact email	bevilacq@usp.br
lab head
Giuseppe Palmisano
contact affiliation	University of Sao Paulo
contact email	palmisano.gp@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/12/PXD025764

PRIDE project URI

• PRIDE
  1. PXD025764
     1. Label: PRIDE project
     2. Name: The impact of Zika virus exposure on the placental proteomic profile