PXD025760

PXD025760 is an original dataset announced via ProteomeXchange.

Title	C.auto_3gas_absquant: Spike-in SIL-protein stock absolute quantification
Description	Microbes that can recycle one-carbon (C1) greenhouse gases into fuels and chemicals are vital for the biosustainability of future industries. Acetogens are the most efficient known microbes for fixing carbon oxides CO2 and CO. Understanding proteome allocation is important for metabolic engineering as it dictates metabolic fitness. Here, we use absolute proteomics to quantify intracellular concentrations for >1,000 proteins in the model-acetogen Clostridium autoethanogenum grown on three gas mixtures. We detect prioritisation of proteome allocation for C1 fixation and significant expression of proteins involved in the production of acetate and ethanol as well as proteins with unclear functions. The data also revealed which isoenzymes are important. Integration of proteomic and metabolic flux data demonstrated that enzymes catalyse high fluxes with high concentrations and high in vivo catalytic rates. We show that flux throughput was dominantly controlled through enzyme catalytic rates rather than concentrations. Our work serves as a reference dataset and advances systems-level understanding and engineering of acetogens.
HostingRepository	PanoramaPublic
AnnounceDate	2022-03-22
AnnouncementXML	Submission_2022-03-22_09:14:16.646.xml
DigitalObjectIdentifier
ReviewLevel	Non peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Kaspar Valgepea
SpeciesList	scientific name: Clostridium autoethanogenum; NCBI TaxID: 84023;
ModificationList	Label:13C(6)15N(2)
Instrument	Q Exactive HF-X

Revision	Datetime	Status	ChangeLog Entry
0	2021-05-03 09:07:40	ID requested
⏵ 1	2022-03-22 09:14:17	announced

Dataset with its publication pending

submitter keyword: absolute quantification, acetogen, gas fermentation, PRM

Esteban Marcellin
contact affiliation	Australian Institute for Bioengineering and Nanotechnology (AIBN), The University of Queensland, Australia
contact email	e.marcellin@uq.edu.au
lab head
Kaspar Valgepea
contact affiliation	Australian Institute for Bioengineering and Nanotechnology (AIBN), The University of Queensland, Australia
contact email	kaspar.valgepea@ut.ee
dataset submitter

Panorama Public dataset URI