PXD025759
PXD025759 is an original dataset announced via ProteomeXchange.
Dataset Summary
Title | A novel method for creating a synthetic L-DOPA proteome and in vitro evidence of incorporation. |
Description | Proteinopathies are protein misfolding diseases that have an underlying factor that effect the conformation of proteoforms. A factor hypothesised to play a role in these diseases is the incorporation of non-protein amino acids into proteins, with a key example being the therapeutic drug levodopa. The presence of levodopa as a protein constituent has been explored in several studies but it has not been examined in a global proteomic manner. This paper provides a proof-of-concept method for enzymatically creating levodopa containing proteins using the enzyme tyrosinase, provides spectral evidence of in vitro incorporation and the induction of the unfolded protein response due to levodopa. |
HostingRepository | PRIDE |
AnnounceDate | 2024-10-07 |
AnnouncementXML | Submission_2024-10-07_13:39:52.925.xml |
DigitalObjectIdentifier | https://dx.doi.org/10.6019/PXD025759 |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Supported dataset by repository |
PrimarySubmitter | Joel Steele |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | carbamoylated residue; L-3',4'-dihydroxyphenylalanine; hydroxylated residue; monohydroxylated residue; deaminated residue |
Instrument | Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
---|---|---|---|
0 | 2021-05-03 00:01:31 | ID requested | |
⏵ 1 | 2024-10-07 13:39:53 | announced |
Publication List
10.6019/PXD025759; |
10.3390/proteomes9020024; |
Steele JR, Strange N, Rodgers KJ, Padula MP, A Novel Method for Creating a Synthetic L-DOPA Proteome and In Vitro Evidence of Incorporation. Proteomes, 9(2):(2021) [pubmed] |
Keyword List
submitter keyword: Misincorporation |
post translational modifications |
PTM |
Levodopa |
L-DOPA |
Contact List
Kenneth J Rodgers | |
---|---|
contact affiliation | Neurotoxin Research Group, School of Life Sciences, The University of Technology Sydney, Ultimo, NSW 2007, Australia; kenneth.rodgers@uts.edu.au (K.J.R.) |
contact email | Kenneth.Rodgers@uts.edu.au |
lab head | |
Joel Steele | |
contact affiliation | University of Technology Sydney |
contact email | joel.r.steele@gmail.com |
dataset submitter |
Full Dataset Link List
Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/02/PXD025759 |
PRIDE project URI |
Repository Record List
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