PXD025757

PXD025757 is an original dataset announced via ProteomeXchange.

Title	A Chemoproteomic Approach toward Probing the Interactomes of Perfluoroalkyl Substances
Description	Poly- and perfluoroalkyl substances (PFASs) are widely used in industrial products and consumer goods. Due to their extreme-ly recalcitrant nature and potential bioaccumulation and toxicity, exposure to PFASs may result in adverse health outcomes in humans and wildlife. In this study, we developed a chemoproteomic strategy, based on the use of isotope-coded desthiobiotin-perfluorooctanephosphonic acid (PFOPA) probe and LC-MS/MS analysis, to profile PFAS-binding proteins. Targeted proteins were labeled with the desthiobiotin-PFOPA probe, digested with trypsin, and the ensuing desthiobiotin-conjugated peptides were enriched with streptavidin beads for LC-MS/MS analysis. We were able to identify 469 putative PFOPA-binding proteins. By conducting competitive binding experiments using a low (10 M) and high (100 M) concentrations of stable isotope-labeled PFOPA probes, we further identified 128 non-redundant peptides derived from 75 unique proteins that exhibit selective binding toward PFOPA. Additionally, we demonstrated that one of these proteins, fatty acid-binding protein 5 (FABP5), could interact directly with PFASs. Furthermore, the desthiobiotin-labeled lysine residues are located close to the fatty acid-binding pocket of FABP5, and the binding affinity varies with the structures of PFASs. Taken together, we developed a novel chemo-proteomic method for interrogating the PFAS-interacting proteome. The identification of these proteins sets the stage for un-derstanding the mechanisms through which exposure to PFASs confers adverse human health effects.
HostingRepository	PRIDE
AnnounceDate	2022-08-12
AnnouncementXML	Submission_2022-08-11_23:56:43.475.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Quanqing Zhang
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2021-05-02 23:59:05	ID requested
⏵ 1	2022-08-11 23:56:44	announced

Zhang Q, Dong X, Lu J, Song J, Wang Y, Chemoproteomic Approach toward Probing the Interactomes of Perfluoroalkyl Substances. Anal Chem, 93(27):9634-9639(2021) [pubmed]

submitter keyword: PFASs, Probe

Quanqing Zhang
contact affiliation	University of California Riverside
contact email	zqq84490411@gmail.com
lab head
Quanqing Zhang
contact affiliation	University of California Riverside
contact email	zqq84490411@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/08/PXD025757

PRIDE project URI

• PRIDE
  1. PXD025757
     1. Label: PRIDE project
     2. Name: A Chemoproteomic Approach toward Probing the Interactomes of Perfluoroalkyl Substances