PXD025751

PXD025751 is an original dataset announced via ProteomeXchange.

Title	Effect of soybean agglutinin on biological function of gut
Description	This study aims to investigate the effects of SBA on the cell cycle, apoptosis, and to verify the mechanism of SBA anti-nutritional characters based on proteomic-based analysis. The IPEC-J2 cell line was cultured with medium containing 0.0, 0.5 or 2.0 mg/mL SBA. With increasing SBA levels, the percentage of the cells at G0/G1 phase, cell apoptosis rates, expressions of Bax and p21, and the activities of Casp-3 and Casp-9 were increased, while cyclin D1 and Bcl-2 expressions were declined (p<0.05). The proteomic analysis showed that the numbers of differentially expressed proteins, induced by SBA, were mainly enriched in different pathways including DNA replication, base excision repair, nucleus excision repair, mismatch repair, amide and peptide biosynthesis, ubiquitin-mediated proteolysis, as well as structures and functions of mitochondria and ribosome. In conclusion, the anti-nutritional mechanism of SBA is a complex cellular process. Such process including DNA related activities; protein synthesis and metabolism; signal-conducting relation; as well as subcellular structure and function. This study provides comprehensive information to understand the toxic mechanism of SBA in monogastrics.
HostingRepository	PRIDE
AnnounceDate	2021-05-04
AnnouncementXML	Submission_2021-06-21_22:28:57.354.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Mohammed Hamdy Farouk
SpeciesList	scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702; scientific name: Vitis vinifera (Grape); NCBI TaxID: 29760; scientific name: Candida albicans (Yeast); NCBI TaxID: 5476; scientific name: Acyrthosiphon pisum (Pea aphid); NCBI TaxID: 7029; scientific name: Equus caballus (Horse); NCBI TaxID: 9796; scientific name: Mesocricetus auratus (Golden hamster); NCBI TaxID: 10036; scientific name: Rattus rattus (Black rat); NCBI TaxID: 10117; scientific name: Bombyx mori (Silk moth); NCBI TaxID: 7091; scientific name: Caenorhabditis elegans; NCBI TaxID: 6239; scientific name: Solanum tuberosum (Potato); NCBI TaxID: 4113; scientific name: Hepatitis C virus subtype 1a; NCBI TaxID: 31646; scientific name: Bos taurus (Bovine); NCBI TaxID: 9913; scientific name: Trypanosoma brucei; NCBI TaxID: 5691; scientific name: Danio rerio (Zebrafish) (Brachydanio rerio); NCBI TaxID: 7955; scientific name: Drosophila melanogaster (Fruit fly); NCBI TaxID: 7227; scientific name: Helicobacter pylori J99 (Campylobacter pylori J99); NCBI TaxID: 85963; scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; scientific name: Merluccius bilinearis (Silver hake); NCBI TaxID: 79698; scientific name: Brassica oleracea var. botrytis (Cauliflower); NCBI TaxID: 3715; scientific name: Yarrowia lipolytica (Candida lipolytica); NCBI TaxID: 4952; scientific name: Pongo pygmaeus (Bornean orangutan); NCBI TaxID: 9600; scientific name: Cercopithecus aethiops (Green monkey) (Grivet); NCBI TaxID: 9534; scientific name: Pisum sativum (Garden pea); NCBI TaxID: 3888; scientific name: Heliocidaris erythrogramma (Sea urchin); NCBI TaxID: 7634; scientific name: Rattus norvegicus (Rat); NCBI TaxID: 10116; scientific name: Staphylococcus aureus; NCBI TaxID: 1280; scientific name: Nicotiana tabacum (Common tobacco); NCBI TaxID: 4097; scientific name: Xenopus laevis (African clawed frog); NCBI TaxID: 8355; scientific name: Dictyostelium discoideum (Slime mold); NCBI TaxID: 44689; scientific name: Macaca mulatta (Rhesus macaque); NCBI TaxID: 9544; scientific name: Pinus radiata (Monterey pine); NCBI TaxID: 3347; scientific name: Hordeum vulgare (Barley); NCBI TaxID: 4513; scientific name: Olea europaea (Common olive); NCBI TaxID: 4146; scientific name: Pan troglodytes (Chimpanzee); NCBI TaxID: 9598; scientific name: Arachis hypogaea (Peanut); NCBI TaxID: 3818; scientific name: Brassica napus (Rape); NCBI TaxID: 3708; scientific name: Ricinus communis (Castor bean); NCBI TaxID: 3988; scientific name: Vigna mungo (Rice bean) (Black gram); NCBI TaxID: 3915; scientific name: Ictalurus punctatus (Channel catfish); NCBI TaxID: 7998; scientific name: Taeniopygia guttata (Zebra finch) (Poephila guttata); NCBI TaxID: 59729; scientific name: Enterococcus faecalis (Streptococcus faecalis); NCBI TaxID: 1351; scientific name: Triticum aestivum (Wheat); NCBI TaxID: 4565; scientific name: Homo sapiens (Human); NCBI TaxID: 9606; scientific name: Halobacterium salinarium (Halobacterium halobium); NCBI TaxID: 2242; scientific name: Cicer arietinum (Chickpea) (Garbanzo); NCBI TaxID: 3827; scientific name: Sus scrofa domesticus (domestic pig); NCBI TaxID: 9825; scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932; scientific name: Escherichia coli; NCBI TaxID: 562; scientific name: Apis mellifera (Honeybee); NCBI TaxID: 7460; scientific name: Gallus gallus (Chicken); NCBI TaxID: 9031; scientific name: Oryza sativa (Rice); NCBI TaxID: 4530; scientific name: Zea mays (Maize); NCBI TaxID: 4577; scientific name: Gadus morhua (Atlantic cod); NCBI TaxID: 8049; scientific name: Struthio camelus (Ostrich); NCBI TaxID: 8801;
ModificationList	carbamoylated residue; acetylated residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2021-05-02 23:31:35	ID requested
1	2021-05-04 00:31:30	announced
⏵ 2	2021-06-21 22:28:58	announced	2021-06-22: Updated publication reference for PubMed record(s): 34150879.

Pan L, Liu Y, Lan H, Bao N, Zhao Y, Sun H, Qin G, Farouk MH, Biological Mechanisms Induced by Soybean Agglutinin Using an Intestinal Cell Model of Monogastric Animals. Front Vet Sci, 8():639792(2021) [pubmed]

ProteomeXchange project tag: Toxicoproteomics (B/D-HPP), Food and Nutrition (B/D-HPP)

submitter keyword: SBA, Gut

Guixin Qin
contact affiliation	Key Laboratory of Animal Production, Product Quality and Security, Ministry of Education, Key Laboratory of Animal Nutrition and Feed Science, Jilin Province, College of Animal Science and Technology, Jilin Agricultural University, Changchun, P. R. China 130118
contact email	qgx@jlau.edu.cn
lab head
Mohammed Hamdy Farouk
contact affiliation	Animal Production Department, Faculty of Agriculture, Al-Azhar University, Nasr City, Cairo
contact email	mhfarouk@azhar.edu.eg
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/05/PXD025751

PRIDE project URI

• PRIDE
  1. PXD025751
     1. Label: PRIDE project
     2. Name: Effect of soybean agglutinin on biological function of gut