PXD025748

PXD025748 is an original dataset announced via ProteomeXchange.

Title	Longitudinal evaluation of biomarkers in wound fluids of venous leg ulcers treated with a protease-modulating wound dressing
Description	Venous leg ulcers (VLU) represent a clinical challenge and impair the patient’s quality of life. Venous insufficiency is underlying the protracted healing. We report the protein expression pattern for biomarkers in VLU wound fluids and compare these with acute split-thickness wound fluids. Fifty-seven patients with VLU were recruited and treated for 12 weeks with a protease-modulating polyacrylate wound dressing and a two-layer bandage compression system. Ten patients with acute wounds were followed for 21 days. Wound size decreased from 18.7 ± 12.3 cm2 to 11.75 ± 11.39 cm2 in the VLU cohort, 10 wounds healed completely. Relative wound area reduction reached 48.9% ± 51.9% at week 12. 61.4% of the patients achieved a relative wound area reduction of ≥40% and 50.9% a relative wound area reduction of ≥60%. IL-1beta, TNF-alpha, and MMP9 concentration ranges were similar in VLU and acute wound fluids. Concentrations of S100A8, S100A9, neutrophil elastase, MMP2, and fibronectin were elevated in VLU wound fluids and decreased significantly in abundance after two weeks of treatment. The values remained low, yet, higher than those of acute wounds. Collagen (I) alpha1 abundance was higher in VLU wound fluids and not significantly regulated. Our data show a robust healing response in venous leg ulcers treated with a protease-modulating polyacrylate wound dressing. The biomarker pattern in VLU wound fluids changed within the first two weeks and we speculate that this might reflect a decrease of STAT3 influence in the wound bed.
HostingRepository	PanoramaPublic
AnnounceDate	2023-08-22
AnnouncementXML	Submission_2023-08-22_14:05:50.391.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Ulrich auf dem Keller
SpeciesList	scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationList	Carbamidomethyl; Label:13C(6)15N(2); Label:13C(6)15N(4)
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2021-04-30 21:51:13	ID requested
⏵ 1	2023-08-22 14:05:51	announced

Mikosi, ń, ski J, Kalogeropoulos K, Bundgaard L, Larsen CA, Savickas S, Moldt Haack A, Pa, ń, czak K, Rybo, ł, owicz K, Grzela T, Olszewski M, Ciszewski P, Sitek-Zi, ó, ł, kowska K, Twardowska-Saucha K, Karczewski M, Rabczenko D, Segiet A, Buczak-Kula P, Schoof EM, Eming SA, Smola H, Auf dem Keller U, Longitudinal Evaluation of Biomarkers in Wound Fluids from Venous Leg Ulcers and Split-thickness Skin Graft Donor Site Wounds Treated with a Protease-modulating Wound Dressing. Acta Derm Venereol, 102():adv00834(2022) [pubmed]

submitter keyword: protease-modulating wound dressing, protein biomarker, SureQuant, venous leg ulcer, wound fluid

Ulrich auf dem Keller
contact affiliation	Technical University of Denmark
contact email	uadk@dtu.dk
lab head
Ulrich auf dem Keller
contact affiliation	Technical University of Denmark
contact email	uadk@dtu.dk
dataset submitter

Panorama Public dataset URI