PXD025745

PXD025745 is an original dataset announced via ProteomeXchange.

Title	The MICOS-MIB complex member PERM1 connects mitochondria with the sarcolemma via ankyrin B
Description	Skeletal muscle subsarcolemmal mitochondria (SSM) and intermyofibrillar mitochondria subpopulations have distinct metabolic activity and sensitivity, though the mechanisms that localize SSM to peripheral areas of muscle fibers are poorly understood. A protein interaction study and complexome profiling identified PERM1 interacts with the MICOS-MIB complex. Ablation of Perm1 in mice reduced muscle force, decreased mitochondrial membrane potential and complex I activity, and reduced the numbers of SSM in skeletal muscle. We demonstrate PERM1 interacts with the intracellular adaptor protein ankyrin B (ANKB) that connects the cytoskeleton to the plasma membrane. In addition, we discovered a C-terminal transmembrane helix that anchors PERM1 into the outer mitochondrial membrane. We conclude PERM1 functions in the MICOS-MIB complex and acts as an adapter to connect the mitochondria with the sarcolemma via ANKB.
HostingRepository	PRIDE
AnnounceDate	2021-11-16
AnnouncementXML	Submission_2021-11-16_08:10:00.160.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Theresa Bock
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2021-04-30 07:33:34	ID requested
⏵ 1	2021-11-16 08:10:00	announced

Bock T, T, ü, rk C, Aravamudhan S, Keufgens L, Bloch W, Rozsivalova DH, Romanello V, Nogara L, Blaauw B, Trifunovic A, Braun T, Kr, ü, ger M, PERM1 interacts with the MICOS-MIB complex to connect the mitochondria and sarcolemma via ankyrin B. Nat Commun, 12(1):4900(2021) [pubmed]

submitter keyword: Mitochondria

Skeletal muscle

PERM1

Proteomics

Marcus Krüger
contact affiliation	Institute for Genetics, Cologne Excellence Cluster on Cellular Stress Responses in Aging-Associated Diseases (CECAD), Cologne, Germany
contact email	marcus.krueger@uni-koeln.de
lab head
Theresa Bock
contact affiliation	CECAD Research Center, University of Cologne
contact email	tbock2@uni-koeln.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/11/PXD025745

PRIDE project URI

• PRIDE
  1. PXD025745
     1. Label: PRIDE project
     2. Name: The MICOS-MIB complex member PERM1 connects mitochondria with the sarcolemma via ankyrin B