PXD025738

PXD025738 is an original dataset announced via ProteomeXchange.

Title	Pumilio2 and Staufen2 selectively balance the synaptic proteome
Description	Cells, in particular neurons, have the capacity to adapt to environmental stimuli, a phenomenon termed cellular plasticity. The underlying processes are controlled by a network of RNA-binding proteins (RBPs). Their impact on cellular plasticity, however, is largely unknown. To address this important question, we chose Pumilio2 (Pum2) and Staufen2 (Stau2) that both regulate synaptic transmission. Importantly, even though both RBPs dynamically interact with each other in neurons, their respective impact on the transcriptome and proteome is highly selective. While Pum2 deficiency leads to reduced translation and protein expression, Stau2 depletion preferentially impacts RNA levels and increases protein abundance. Furthermore, Pum2 activates expression of key GABAergic synaptic components, e.g. the GABAA receptor scaffold protein Gephyrin. Consequently, Pum2 depletion selectively reduced the amplitude of miniature inhibitory postsynaptic currents. Together, our data demonstrate that RBPs are needed to maintain proteostasis in order to control distinct signaling pathways which critically balance synaptic transmission.
HostingRepository	PRIDE
AnnounceDate	2021-09-14
AnnouncementXML	Submission_2021-09-14_08:17:32.845.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Ignasi Forne
SpeciesList	scientific name: Rattus norvegicus (Rat); NCBI TaxID: 10116;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2021-04-30 04:58:08	ID requested
⏵ 1	2021-09-14 08:17:33	announced

Dataset with its publication pending

submitter keyword: Pumilio2, Staufen2, RNA-binding protein, translation activation, neuronal proteome, neuronal inhibition

Prof Michael Kiebler
contact affiliation	Department for Cell Biology Biomedical Center (BMC) Medical Faculty, LMU Großhaderner Straße 9, 82152 Planegg-Martinsried, Germany
contact email	mkiebler@lmu.de
lab head
Ignasi Forne
contact affiliation	Biomedical Center-LMU
contact email	ignasi.forne@lrz.uni-muenchen.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/06/PXD025738

PRIDE project URI

• PRIDE
  1. PXD025738
     1. Label: PRIDE project
     2. Name: Pumilio2 and Staufen2 selectively balance the synaptic proteome