PXD025716 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Upstream open reading frames regulate proto-oncogene translation and encode HLA-presented immunogenic tumor antigens |
Description | Upstream open reading frames (uORFs) represent translational control elements within eukaryotic transcript leader sequences. Recent data showed that uORFs can encode for biologically active proteins and human leucocyte antigen (HLA)-presented peptides and suggest their potential role in cancer cell development and survival. However, it is so far unclear if uORF-encoded peptides could serve as tumor-associated antigen targets and thus also play a role in cancer immune surveillance. Combining mass spectrometry-based immunopeptidome analysis in primary tumor and healthy tissues and evaluation of proto-oncogene-associated uORF-mediated translational control we here identified a panel of HLA-presented tumor-associated uORF-derived antigens. These uORF-derived tumor antigens were further shown to induce multifunctional antigen-specific T cells, validating their suitability as antigen targets for T cell-based cancer immunotherapy. Our data further unravel the role of uORF-encoded peptides in malignant disease, suggesting uORF-derived tumor-associated antigens as targets for anti-cancer immune surveillance and immunotherapy development. |
HostingRepository | PRIDE |
AnnounceDate | 2022-05-27 |
AnnouncementXML | Submission_2022-05-27_05:08:32.278.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Annika Nelde |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | monohydroxylated residue |
Instrument | Orbitrap Fusion Lumos; LTQ Orbitrap |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2021-04-29 10:50:15 | ID requested | |
⏵ 1 | 2022-05-27 05:08:33 | announced | |
Publication List
Nelde A, Fl, ö, totto L, J, ü, rgens L, Szymik L, Hubert E, Bauer J, Schliemann C, Kessler T, Lenz G, Rammensee HG, Walz JS, Wethmar K, Upstream open reading frames regulate translation of cancer-associated transcripts and encode HLA-presented immunogenic tumor antigens. Cell Mol Life Sci, 79(3):171(2022) [pubmed] |
Keyword List
submitter keyword: uORF, cancer, immunotherapy, immunopeptidomics, HLA peptides |
Contact List
Juliane S. Walz |
contact affiliation | Clinical Collaboration Unit Translational Immunology, German Cancer Consortium (DKTK), Department of Internal Medicine, University Hospital Tübingen, Tübingen, Germany |
contact email | juliane.walz@med.uni-tuebingen.de |
lab head | |
Annika Nelde |
contact affiliation | Clinical Collaboration Unit Translational Immunology, German Cancer Consortium (DKTK), Department of Internal Medicine, University Hospital Tübingen, Germany |
contact email | annika.nelde@uni-tuebingen.de |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/04/PXD025716 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD025716
- Label: PRIDE project
- Name: Upstream open reading frames regulate proto-oncogene translation and encode HLA-presented immunogenic tumor antigens