PXD025702

PXD025702 is an original dataset announced via ProteomeXchange.

Title	Neutrophils from patients with recurring Panton-Valentine Leukocidin-positive Staphylococcus aureus infections are more sensitive to PVL induced NETosis
Description	Panton-Valentine Leukocidin (PVL) is a Staphylococcus aureus toxin that binds to and kills human neutrophils resulting in the formation of neutrophil extracellular traps. A subset of individuals colonized with PVL expressing S. aureus suffer from recurring infections. We found that neutrophils from affected individuals display increased spontaneous NET formation after isolation, and increased sensitivity to killing by PVL. Compared to healthy controls, the expression of the target receptors for PVL, CD45 and C5L2, but not CD88, was increased in these patients, and the expression correlated to the amount of PVL-induced NETs produced. NADPH-oxidase activity was not important for PVL induced NETosis as neutrophils from CGD patients produced NETs in response to PVL. Through NET proteome analysis we identified that the protein content of PVL induced NETs is different from mitogen induced NETs. The abundance of the antimicrobial proteins LL37, myeloperoxidase, azurocidin, and proteinase 3 was lower on PVL NETs and PVL-induced NETs were deficient in killing Staphylococcus aureus. Neutrophils from patients that suffer from recurring PVL-positive infections may be more sensitive to PVL-induced NETosis, impairing their ability to combat the infection.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_06:15:46.889.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Christian Frese
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2021-04-29 01:33:16	ID requested
1	2023-11-23 13:45:40	announced
⏵ 2	2024-10-22 06:15:47	announced	2024-10-22: Updated project metadata.

Jhelum H, Č, erina D, Harbort CJ, Lindner A, Hanitsch LG, Leistner R, Schr, ö, der JT, von Bernuth H, Stegemann MS, Sch, ü, rmann M, Zychlinsky A, Kr, ü, ger R, Marsman G, Panton-Valentine leukocidin-induced neutrophil extracellular traps lack antimicrobial activity and are readily induced in patients with recurrent PVL + -Staphylococcus aureus infections. J Leukoc Biol, 115(2):222-234(2024) [pubmed]

10.1093/jleuko/qiad137;

submitter keyword: Netosis,NETs, MRSA, leukocidin, Staphylococcus aureus, PVL

Christian Karl Frese
contact affiliation	Max Planck Unit for the Science of Pathogens, Berlin, Germany
contact email	frese@mpusp.mpg.de
lab head
Christian Frese
contact affiliation	Max Planck Unit for the Science of Pathogens
contact email	frese@mpusp.mpg.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD025702
     1. Label: PRIDE project
     2. Name: Neutrophils from patients with recurring Panton-Valentine Leukocidin-positive Staphylococcus aureus infections are more sensitive to PVL induced NETosis