PXD025700

PXD025700 is an original dataset announced via ProteomeXchange.

Title	A hierarchical transcriptional network controls appressorium-1 mediated plant 2 infection by the rice blast fungus Magnaporthe oryzae
Description	Rice blast is a pervasive and devastating disease that threatens rice production across the world. In spite of its importance to global food security, however, the underlying biology of plant infection by the blast fungus Magnaporthe oryzae remains poorly understood. In particular, it is unclear how the fungus elaborates a specialised infection cell, the appressorium, in response to surface signals from the rice leaf. Here, we report the identification of a network of temporally co-regulated transcription factors that act downstream of the Pmk1 mitogen-activated protein kinase pathway to regulate gene expression during appressorium-mediated plant infection. We have functionally characterised this network of transcription factors and demonstrated the operation of a hierarchical transcriptional control system. We show that this tiered regulatory mechanism involves Pmk1-dependent phosphorylation of the Hox7 homeobox transcription factor, which represses hyphal-associated gene expression and simultaneously induces major physiological changes required for appressorium development, including cell cycle arrest, autophagic cell death, turgor generation and melanin biosynthesis. Mst12 then regulates gene functions involved in septin-dependent cytoskeletal re-organisation, polarised exocytosis and effector gene expression necessary for plant tissue invasion.4929
HostingRepository	PRIDE
AnnounceDate	2022-04-13
AnnouncementXML	Submission_2022-04-13_03:36:10.615.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD025700
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	FRank Menke
SpeciesList	scientific name: Escherichia coli; NCBI TaxID: 562; scientific name: Magnaporthe sp. MG05; NCBI TaxID: 1758316;
ModificationList	phosphorylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2021-04-29 01:33:15	ID requested
⏵ 1	2022-04-13 03:36:11	announced

Os, é, s-Ruiz M, Cruz-Mireles N, Martin-Urdiroz M, Soanes DM, Eseola AB, Tang B, Derbyshire P, Nielsen M, Cheema J, Were V, Eisermann I, Kershaw MJ, Yan X, Valdovinos-Ponce G, Molinari C, Littlejohn GR, Valent B, Menke FLH, Talbot NJ, Appressorium-mediated plant infection by Magnaporthe oryzae is regulated by a Pmk1-dependent hierarchical transcriptional network. Nat Microbiol, 6(11):1383-1397(2021) [pubmed]

submitter keyword: MAP kinase, Magnaporthe oryzae, Transcription, Phosphorylation, Rice blast, LC-MS/MS

Frank Menke
contact affiliation	frank.menke@tsl.ac.uk
contact email	frank.menke@tsl.ac.uk
lab head
FRank Menke
contact affiliation	the Sainsbury laboratory
contact email	Frank.Menke@tsl.ac.uk
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD025700
     1. Label: PRIDE project
     2. Name: A hierarchical transcriptional network controls appressorium-1 mediated plant 2 infection by the rice blast fungus Magnaporthe oryzae