PXD025697
PXD025697 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Proteomics reveals Steap3-Gm2a axis controlling lysosomal escape of Listeria monocytogenes in murine macrophages |
| Description | As a pathogenic microorganism, Listeria monocytogenes is widely used in the study of bacterial pathogenic mechanism and host defense mechanism. The lysosomal escape of Listeria monocytogenes is essential for its replication in the cytoplasm. Here, we report that Listeria monocytogenes infection can cause the expression level of Six-transmembrane epithelial antigen of the prostate 3 (Steap3) to decrease in macrophages. Functional experiments show that Steap3 can inhibit lysosomal escape of Listeria monocytogenes. Then, the comprehensive proteomic analysis reveals that the deletion of Steap3 affects the lysosomal signaling pathway of macrophages. Among these proteins affected by Steap3, we discover that Ganglioside GM2 activator (Gm2a) may be a potential target gene of Steap3. In summary, we find that Steap3-Gm2a axis can inhibits lysosomal escape of Listeria monocytogenes and serve the potential molecular drug targets for antibacterial treatment. |
| HostingRepository | iProX |
| AnnounceDate | 2021-04-29 |
| AnnouncementXML | Submission_2021-05-12_19:17:38.639.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Jiangbei Yuan |
| SpeciesList | scientific name: Mus musculus; NCBI TaxID: 10090; |
| ModificationList | iodoacetamide derivatized residue |
| Instrument | LTQ Orbitrap |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2021-04-28 21:46:46 | ID requested | |
| ⏵ 1 | 2021-05-12 19:17:39 | announced |
Publication List
| Dataset with its publication pending |
Keyword List
| submitter keyword: Steap3, Listeria monocytogenes infection, Proteomics, Gm2a, Mass spectrometry, lysosomal escape |
Contact List
| Jiangbei Yuan | |
|---|---|
| contact affiliation | Peking University Shenzhen Hospital |
| contact email | yuanjiangbei@163.com |
| lab head | |
| Jiangbei Yuan | |
| contact affiliation | Peking University Shenzhen Hospital |
| contact email | yuanjiangbei@163.com |
| dataset submitter | |
Full Dataset Link List
| iProX dataset URI |
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