PXD025693

PXD025693 is an original dataset announced via ProteomeXchange.

Title	Exposure of Candida parapsilosis to the silver(I) compound SBC3 induces alterations in the proteome inhibiting protein synthesis and reducing virulence
Description	The antimicrobial properties of silver have been exploited for many centuries and continue to gain interest in the fight against antimicrobial drug resistance. The broad-spectrum activity and low toxicity of silver has led to its incorporation into a wide range of novel antimicrobial agents, including N-heterocyclic carbene (NHC) complexes. These versatile organic compounds readily bind transition metals and allow various substitutions, ring sizes and general properties. The antimicrobial activity and in vivo efficacy of the NHC silver(I) acetate complex SBC3, derived from 1,3-dibenzyl-4,5-diphenylimidazol-2-ylidene (NHC*), has previously been demonstrated, although the mode(s) of action of SBC3 remained unclear. Label-free quantitative (LFQ) proteomics was employed to analyse protein abundance changes in the yeast Candida parapsilosis in response to SBC3 treatment and gain insight into potential SBC3 targets. Proteomic analysis of C. parapsilosis following exposure to SBC3 revealed increased abundance in proteins associated with cell wall synthesis, detoxification and drug efflux that are indicative of a cell stress response. Significant decreases in proteins required for protein synthesis, amino acid biosynthesis and respiration offer potential insight into the growth-inhibitory mechanisms of SBC3. Exposure of C. parapsilosis to SBC3 lead to reduced adherence to epithelial cells and biofilm formation which may indicate the compound can reduce fungal virulence.
HostingRepository	PRIDE
AnnounceDate	2022-06-28
AnnouncementXML	Submission_2022-06-28_08:51:17.460.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Magdalena Piatek
SpeciesList	scientific name: Candida parapsilosis CDC317; NCBI TaxID: 578454;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2021-04-28 07:40:05	ID requested
⏵ 1	2022-06-28 08:51:18	announced

Piatek M, O'Beirne C, Beato Z, Tacke M, Kavanagh K, Exposure of Candida parapsilosis to the silver(I) compound SBC3 induces alterations in the proteome and reduced virulence. Metallomics, 14(8):(2022) [pubmed]

submitter keyword: antimicrobial, silver, Candida, proteomics

Kevin Kavanagh
contact affiliation	Medical Mycology Laboratory, SSPC Pharma Research Centre, Biology Department, Maynooth University, Co. Kildare, Ireland.
contact email	kevin.kavanagh@mu.ie
lab head
Magdalena Piatek
contact affiliation	Maynooth University
contact email	magdalena.piatek@mu.ie
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD025693
     1. Label: PRIDE project
     2. Name: Exposure of Candida parapsilosis to the silver(I) compound SBC3 induces alterations in the proteome inhibiting protein synthesis and reducing virulence