PXD025681

PXD025681 is an original dataset announced via ProteomeXchange.

Title	Proteomic analysis of Shewanella oneidensis overexpressing or not the putrescin decarboxylase speC
Description	Some microorganisms can respire with extracellular electron acceptors using an extended electron transport chain to the cell surface. These organisms apply flavin molecules as cofactors to facilitate one-electron transfer catalysed by the terminal reductases and as endogenous electron shuttles. In the model organism Shewanella oneidensis, riboflavin production and excretion triggers a specific biofilm formation response that is initiated at a specific threshold concentration, similar to canonical quorum sensing molecules. Riboflavin-mediated messaging is based on the overexpression of the gene encoding the putrescin decarboxylase speC which leads to posttranscriptional overproduction of proteins involved in biofilm formation. We performed a mass spectrometry-based analysis of cells with and without speC overexpression to identify the effect of SpeC overexpression on the cell proteome.
HostingRepository	PRIDE
AnnounceDate	2021-07-16
AnnouncementXML	Submission_2021-07-15_23:52:31.453.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD025681
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Yohann Couté
SpeciesList	scientific name: Shewanella oneidensis MR-1; NCBI TaxID: 211586;
ModificationList	monohydroxylated residue; acetylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2021-04-28 02:14:11	ID requested
⏵ 1	2021-07-15 23:52:32	announced

Edel M, Sturm G, Sturm-Richter K, Wagner M, Ducassou JN, Cout, é Y, Horn H, Gescher J, Extracellular riboflavin induces anaerobic biofilm formation in Shewanella oneidensis. Biotechnol Biofuels, 14(1):130(2021) [pubmed]

submitter keyword: Shewanella oneidensis, riboflavin production, biofilm formation, putrescin decarboxylase speC, nanoLC-MS/MS

Virginie Brun
contact affiliation	EDyP
contact email	virginie.brun@cea.fr
lab head
Yohann Couté
contact affiliation	EDyP
contact email	yohann.coute@cea.fr
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/07/PXD025681

PRIDE project URI

• PRIDE
  1. PXD025681
     1. Label: PRIDE project
     2. Name: Proteomic analysis of Shewanella oneidensis overexpressing or not the putrescin decarboxylase speC