PXD025680 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Proteomics informed by transcriptomics for a qualitative and quantitative analysis of the sialoproteome of Ornithodoros moubata adult ticks. SWATH Label Free quantitation |
Description | The specific aim of the present work is to expand the data on the salivary proteome of O. moubata adult ticks, particularly of female ticks, by solving the aforementioned drawbacks, as part of the in depth characterization and analysis of the O. moubata sialome. Knowing this sialome will allow identifying and selecting novel salivary antigens as targets for tick vaccines, and their subsequent testing in animal immunization trials. We pay special attention to female ticks because they are a key developmental stage in the tick life cycle and vaccines may exert on them double deleterious effects, for instance an increase of mortality and a reduction/inhibition of the reproductive performance. With that aim, in the current work we have analysed samples of female and male saliva separately using two different mass spectrometry approaches: data-dependent acquisition (DDA) LC-MS/MS and Sequential Window Acquisition of all Theoretical fragment ion spectra Mass Spectrometry (SWATH MS). This last technique is a specific variant of data-independent acquisition (DIA) methods and is emerging as a technology that combines deep proteome coverage capabilities with quantitative consistency and accuracy. |
HostingRepository | PRIDE |
AnnounceDate | 2021-11-18 |
AnnouncementXML | Submission_2021-11-18_07:08:52.639.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Luz Valero |
SpeciesList | scientific name: Ornithodoros moubata; NCBI TaxID: 6938; |
ModificationList | No PTMs are included in the dataset |
Instrument | TripleTOF 6600 |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2021-04-28 02:14:11 | ID requested | |
⏵ 1 | 2021-11-18 07:08:53 | announced | |
Publication List
Oleaga A, Carnero-Mor, á, n A, Valero ML, P, é, rez-S, á, nchez R, Proteomics informed by transcriptomics for a qualitative and quantitative analysis of the sialoproteome of adult Ornithodoros moubata ticks. Parasit Vectors, 14(1):396(2021) [pubmed] |
Keyword List
submitter keyword: LC MSMS, Label free quantitaion, DIA, SWATH, Ornithodoros moubata proteome |
Contact List
Ana Oleaga Pérez |
contact affiliation | Parasitology Laboratory, Institute of Natural Resources and Agrobiology (IRNASA, CSIC). Cordel de Merinas, 40-52, 37008 Salamanca, Spain. |
contact email | ana.oleaga@irnasa.csic.es |
lab head | |
Luz Valero |
contact affiliation | Universitat de Valencia |
contact email | mluz.valero@uv.es |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD025680
- Label: PRIDE project
- Name: Proteomics informed by transcriptomics for a qualitative and quantitative analysis of the sialoproteome of Ornithodoros moubata adult ticks. SWATH Label Free quantitation