PXD025658

PXD025658 is an original dataset announced via ProteomeXchange.

Title	Proteomics informed by transcriptomics for a qualitative and quantitative analysis of the sialoproteome of Ornithodoros moubata adult ticks. DDA Label free quantitation
Description	The specific aim of the present work is to expand the data on the salivary proteome of O. moubata adult ticks, particularly of female ticks, by solving the aforementioned drawbacks, as part of the in depth characterization and analysis of the O. moubata sialome. Knowing this sialome will allow identifying and selecting novel salivary antigens as targets for tick vaccines, and their subsequent testing in animal immunization trials. We pay special attention to female ticks because they are a key developmental stage in the tick life cycle and vaccines may exert on them double deleterious effects, for instance an increase of mortality and a reduction/inhibition of the reproductive performance. With that aim, in the current work we have analysed samples of female and male saliva separately using two different mass spectrometry approaches: data-dependent acquisition (DDA) LC-MS/MS and Sequential Window Acquisition of all Theoretical fragment ion spectra Mass Spectrometry (SWATH MS). This last technique is a specific variant of data-independent acquisition (DIA) methods and is emerging as a technology that combines deep proteome coverage capabilities with quantitative consistency and accuracy.
HostingRepository	PRIDE
AnnounceDate	2021-11-30
AnnouncementXML	Submission_2021-11-30_13:44:21.689.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Luz Valero
SpeciesList	scientific name: Ornithodoros moubata; NCBI TaxID: 6938;
ModificationList	No PTMs are included in the dataset
Instrument	TripleTOF 6600

Revision	Datetime	Status	ChangeLog Entry
0	2021-04-27 08:33:26	ID requested
⏵ 1	2021-11-30 13:44:22	announced

Oleaga A, Carnero-Mor, á, n A, Valero ML, P, é, rez-S, á, nchez R, Proteomics informed by transcriptomics for a qualitative and quantitative analysis of the sialoproteome of adult Ornithodoros moubata ticks. Parasit Vectors, 14(1):396(2021) [pubmed]

submitter keyword: LC MSMS, DDA, Ornithodoros moubata proteome, Label free quantitation

Ana Oleaga Pérez
contact affiliation	Parasitology Laboratory, Institute of Natural Resources and Agrobiology (IRNASA, CSIC). Cordel de Merinas, 40-52, 37008 Salamanca, Spain.
contact email	ana.oleaga@irnasa.csic.es
lab head
Luz Valero
contact affiliation	Universitat de Valencia
contact email	mluz.valero@uv.es
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/11/PXD025658

PRIDE project URI

• PRIDE
  1. PXD025658
     1. Label: PRIDE project
     2. Name: Proteomics informed by transcriptomics for a qualitative and quantitative analysis of the sialoproteome of Ornithodoros moubata adult ticks. DDA Label free quantitation