PXD025646

PXD025646 is an original dataset announced via ProteomeXchange.

Title	Serum Peptidome: Diagnostic Window into Pathogenic Processes following Occupational Exposure to Carbon Nanomaterials
Description	Growing industrial use of carbon nanotubes and nanofibers (CNT/F) warrants consideration of human health outcomes. CNT/F produces pulmonary, cardiovascular, and other toxic effects in animals, with significant augmentation of bioactive circulating peptides, the serum peptidome. While epidemiology among CNT/F workers reports few acute symptoms, there remains concern that CNT/F may prime for chronic disease. Here, the serum peptidome is assessed for its biomarker potential in detecting sub-symptomatic pathobiology among CNT/F workers using label-free data-independent mass spectrometry. Using a stratified design between High (>0.5 ug/m3) and Low (<0.1 ug/m3) CNT/F exposures, 2,726 serum peptides significantly distinguish the groups. Of these, 41 are found to be highly discriminatory after model building and exhibit a strong linear correlation with personal CNT/F exposures. The top-five peptide model offers ideal prediction with high accuracy (Q2=0.99916). Unsupervised validation affirms 43.5% of the serum peptidomic variance is attributable to CNT/F exposure. Peptide sequence identification reveals a predominant association with vascular pathology. ARHGAP21, ADAM15 and PLPP3 peptides suggest heightened cardiovasculature permeability and F13A1, FBN1 and VWDE peptides infer a pro-thrombotic state among High CNT/F workers. The serum peptidome affords a diagnostic window into pre-symptomatic pathology among CNT/F exposed workers for longitudinal monitoring health risks.
HostingRepository	MassIVE
AnnounceDate	2023-11-20
AnnouncementXML	Submission_2023-11-20_08:23:01.090.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Andrew K Ottens
SpeciesList	scientific name: Homo sapiens; common name: human; NCBI TaxID: 9606;
ModificationList	Sulfo; Phospho; Acetyl
Instrument	SYNAPT G2-Si

Revision	Datetime	Status	ChangeLog Entry
0	2021-04-26 11:55:14	ID requested
⏵ 1	2023-11-20 08:23:01	announced

Mostovenko E, Dahm MM, Schubauer-Berigan MK, Eye T, Erdely A, Young TL, Campen MJ, Ottens AK, Serum peptidome: diagnostic window into pathogenic processes following occupational exposure to carbon nanomaterials. Part Fibre Toxicol, 18(1):39(2021) [pubmed]

submitter keyword: carbon nanotubes, biomarkers, peptidomics, vascular, health outcomes

Andrew K Ottens
contact affiliation	Virginia Commonwealth University
contact email	akottens@vcu.edu
lab head
Andrew K Ottens
contact affiliation	Virginia Commonwealth University
contact email	akottens@vcu.edu
dataset submitter

MassIVE dataset URI

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