PXD025644

PXD025644 is an original dataset announced via ProteomeXchange.

Title	Multiomic analysis of the Arabian camel (Camelus dromedarius) kidney reveals a role for cholesterol in water conservation
Description	The one-humped Arabian camel (Camelus dromedarius) is the most important livestock animal in arid and semi-arid regions and continues to provide basic necessities to millions of people. In the current context of global warming, there is renewed interest in the adaptive mechanisms that enable camelids to survive in arid conditions. Recent investigations described genomic signatures that revealed evolutionary adaptations to desert environments. We now present a comprehensive catalogue of the transcriptomes and proteomes of the dromedary kidney and describe how the gene expression profiles of Differentially Expressed Genes (DEGs) are modulated as a consequence of chronic dehydration and subsequent acute rehydration. We performed RNAseq and quantification of peptides in samples from 15 dromedaries (5 controls, 5 dehydrated and 5 rehydrated). Gene Ontology analyses suggested an enrichment of the cholesterol biosynthetic process and an overrepresentation of categories related to “ion transmembrane transport” in the camel kidney, and RTN analyses confirmed alterations in the transcriptional machinery involved in cholesterol synthesis. These data were validated by RT-qPCR. Based on our hypothesis of a role for cholesterol during dehydration, we identified DEGs with roles in the countercurrent multiplication process which are affected by changes in the level of cholesterol. Thus, we further validated 3 genes coding for ion transporting proteins (KCNJ8, SLC9A7 and ATP1B3) and AQP2, which were upregulated during dehydration. Our datasets suggest that suppression of cholesterol biosynthesis may facilitate water retention in the kidney of the dromedary by indirectly enhancing the osmotic gradient along the medullary interstitium and the AQP2-mediated water reabsorption.
HostingRepository	PRIDE
AnnounceDate	2021-06-21
AnnouncementXML	Submission_2021-06-28_06:11:15.489.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Fernando Alvira Iraizoz
SpeciesList	scientific name: Camelus dromedarius (Dromedary) (Arabian camel); NCBI TaxID: 9838;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2021-04-26 07:00:57	ID requested
1	2021-06-20 22:47:39	announced
⏵ 2	2021-06-28 06:11:16	announced	2021-06-28: Updated publication reference for PubMed record(s): 34163009.

Alvira-Iraizoz F, Gillard BT, Lin P, Paterson A, Pau, ž, a AG, Ali MA, Alabsi AH, Burger PA, Hamadi N, Adem A, Murphy D, Greenwood MP, Multiomic analysis of the Arabian camel (Camelus dromedarius) kidney reveals a role for cholesterol in water conservation. Commun Biol, 4(1):779(2021) [pubmed]

submitter keyword: Desert physiology,Camelus dromedarius,Dromedary camel, Kidney transcriptomics, Countercurrent multiplication, Kidney proteomics, Water deprivation

David Murphy
contact affiliation	Molecular Neuroendocrinology group, Bristol Medical school, University of Bristol
contact email	d.murphy@bristol.ac.uk
lab head
Fernando Alvira Iraizoz
contact affiliation	Bristol Medical School, University of Bristol
contact email	f.alvirairaizoz@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/06/PXD025644

PRIDE project URI

• PRIDE
  1. PXD025644
     1. Label: PRIDE project
     2. Name: Multiomic analysis of the Arabian camel (Camelus dromedarius) kidney reveals a role for cholesterol in water conservation