PXD025642

PXD025642 is an original dataset announced via ProteomeXchange.

Title	Proteomic Responses of Contrasting Alfalfa (Medicago sativa L.)Varieties to High Temperature Stress
Description	Two alfalfa varieties contrasting in heat tolerance, MS30 and MS37, were used for this experiment, and their seeds were provided by Sichuan Academy of Grassland Sciences. After two months of growth, both alfalfa varieties grow into adult-plants. Select 10-15 healthy plants with consistent growth to remain in each nutritional bowl. All materials are transferred to artificial climate boxes for high temperature stress treatment. The light cycle is still the day/night cycle:16/8h. According to the results of a prior experiment and based on the relevant report, the treatment temperature was set to 20℃, 25℃, 30℃, 35℃, 40℃ and 4℃, which is a gradient upward trend. Among them, alfalfa growing at 20°C were used as the control (non-HT stress). All alfalfa varieties were treated at each temperature gradient for 7 days and the leaves was collected for the determination of physiological indicators. Moreover, the physiological conditions of the plants and the humidity of the climate box were observed at any time. water the plants properly once a day, and the alfalfa special fertilizer was applied once in each cycle of temperature stress treatment to ensure the water and nutrition demand of the plant. Based on the results of the above experiment, some physiological responses demonstrated that most alfalfa varieties showed the most significant variation at 20℃, 35℃ and 43℃. Therefore, leaf samples collected from plants after exposure to the rising temperature 7 days were harvested for further TMT quantitative proteomic analysis.
HostingRepository	PRIDE
AnnounceDate	2022-02-17
AnnouncementXML	Submission_2022-02-17_02:57:18.708.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Xinrui Li
SpeciesList	scientific name: Medicago sativa; NCBI TaxID: 3879;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive Plus

Revision	Datetime	Status	ChangeLog Entry
0	2021-04-26 04:46:32	ID requested
⏵ 1	2022-02-17 02:57:19	announced

Li Y, Li X, Zhang J, Li D, Yan L, You M, Zhang J, Lei X, Chang D, Ji X, An J, Li M, Bai S, Yan J, L.) Varieties to High Temperature Stress. Front Plant Sci, 12():753011(2021) [pubmed]

submitter keyword: alfalfa

leaf

high temperature stress

isobaric tandem mass tag labeling (TMT)

Xinrui li
contact affiliation	Sichuan Academy of Grassland Sciences
contact email	lxrpratas@163.com
lab head
Xinrui Li
contact affiliation	Sichuan Academy of Grassland Sciences
contact email	lxrpratas@163.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD025642
     1. Label: PRIDE project
     2. Name: Proteomic Responses of Contrasting Alfalfa (Medicago sativa L.)Varieties to High Temperature Stress