PXD025634

PXD025634 is an original dataset announced via ProteomeXchange.

Title	Cell Data-Indenpendent acquisition LC-MS/MS
Description	In this work, a microwell-chip was prepared and modified. The microwell-chip was used for extraction of metabolites and subsequent protein digestion. Next, direct electrospray ionization mass spectrometry (ESI-MS) was adopted for metabolome identification and a data independent acquisition (DIA)-MS approach was established for simultaneous proteome profiling and phosphoproteome analysis. In particular, application of this strategy provides a multi-omics view of cellular changes.
HostingRepository	PRIDE
AnnounceDate	2021-11-08
AnnouncementXML	Submission_2021-11-08_09:00:01.892.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Yuanyuan Li
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	phosphorylated residue; acetylated residue; deamidated residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2021-04-26 01:30:19	ID requested
⏵ 1	2021-11-08 09:00:02	announced

Li Y, Li H, Xie Y, Chen S, Qin R, Dong H, Yu Y, Wang J, Qian X, Qin W, An Integrated Strategy for Mass Spectrometry-Based Multiomics Analysis of Single Cells. Anal Chem, 93(42):14059-14067(2021) [pubmed]

submitter keyword: Cell, DIA, LC-MS/MS

Weijie Qin
contact affiliation	National Center for Protein Sciences Beijing, State Key Laboratory of Proteomics, Beijing Proteome Research Center, Beijing Institute of Lifeomics, Beijing
contact email	aunp_dna@126.com
lab head
Yuanyuan Li
contact affiliation	National center for protein sciences(Beijing)
contact email	mcircle0329@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/11/PXD025634

PRIDE project URI

• PRIDE
  1. PXD025634
     1. Label: PRIDE project
     2. Name: Cell Data-Indenpendent acquisition LC-MS/MS