PXD025633 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | The The Secretome of Human Neonatal Mesenchymal Stem Cells Modulates Doxorubicin-induced CytotoxicitySecretome of Human Neonatal Mesenchymal Stem Cells Modulates Doxorubicin-induced Cytotoxicity |
Description | Breast cancer remains one of the leading causes of death in women, being the chemotherapeutic agent doxorubicin (Dox) among the most widely standard chemotherapy options for its treatment. However, its effective use has been severely limited owing to its well-documented cardiotoxic side effect that can lead to heart failure in a subset of patients. We have previously shown that a specific population of mesenchymal stem cells (MSCs) present immunomodulatory and regenerative properties, mainly granted by its secretome (CM), whose potential was improved when produced under 3D conditions. In cancer, the role of MSCs is still contradictory, with literature reporting both cancer promoting and suppressive effects. Therefore, we aimed at determining the effect of concomitant exposure of Dox with CM from 3D (CM3D) and 2D (CM2D) MSC cultures in breast cancer cells (MDA-MB-231) and in non-tumour breast epithelial cells (MCF10A) and differentiated AC16 cardiomyocytes. As such, the whole secretome was obtained by collecting and concentrating the culture media conditioned by MSCs in 2D (CM2D) and 3D (CM3D). A Ge-LC-MS/MS proteomic analysis revealed that CM3D effects may be linked to MSC cytoprotection and tumour development, namely through regulation of cell proliferation (CAPN1, CST1, LAMC2, RANBP3), migration (CCN3, MMP-8, PDCD5), invasion (TIMP-1/2), oxidative stress (AIFM1, CD9, GSR) and inflammation (ANXA5, CDH13, GDF-15). Overall, MSC-derived CM3D decreased Dox-induced cytotoxic effects on non-tumour breast cells and cardiomyocytes, without compromising Dox chemotherapeutic nature, highlighting the potential use of CM3D as an adjuvant in chemotherapy to reduce off-target side effects. |
HostingRepository | PRIDE |
AnnounceDate | 2023-11-14 |
AnnouncementXML | Submission_2023-11-14_08:37:01.562.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Rui Vitorino |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | monohydroxylated residue; deamidated residue; iodoacetamide derivatized residue |
Instrument | Q Exactive HF |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2021-04-26 01:22:10 | ID requested | |
1 | 2023-03-10 20:29:04 | announced | |
⏵ 2 | 2023-11-14 08:37:03 | announced | 2023-11-14: Updated project metadata. |
Publication List
Cam, õ, es SP, Bulut O, Yazar V, Gaspar MM, Sim, õ, es S, Ferreira R, Vitorino R, Santos JM, Gursel I, Miranda JP, 3D-MSCs A151 ODN-loaded exosomes are immunomodulatory and reveal a proteomic cargo that sustains wound resolution. J Adv Res, 41():113-128(2022) [pubmed] |
Keyword List
submitter keyword: human |
breast cancer |
proteome |
mesenchymal stem/stromal cells |
secretome |
3D cultures |
Contact List
Joana P Miranda |
contact affiliation | Research Institute for Medicines (iMed.ULisboa), Faculty of Pharmacy, Universidade de Lisboa, Lisbon, Portugal. |
contact email | jmiranda@ff.ul.pt |
lab head | |
Rui Vitorino |
contact affiliation | Universidade de Aveiro |
contact email | rvitorino@ua.pt |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD025633
- Label: PRIDE project
- Name: The The Secretome of Human Neonatal Mesenchymal Stem Cells Modulates Doxorubicin-induced CytotoxicitySecretome of Human Neonatal Mesenchymal Stem Cells Modulates Doxorubicin-induced Cytotoxicity