PXD025632

PXD025632 is an original dataset announced via ProteomeXchange.

Title	Analysis of cryopreservation of human granulosa cells
Description	Human primary granulosa cells (GCs) derived from women, undergoing oocyte retrieval, can be cultured and are a cellular model for the study of human ovarian functions. In vitro they change rapidly, resembling initially cells of the preovulatory follicle and then cells of the corpus luteum. They are derived from individual patients and their different medical history, lifestyle and age lead to heterogeneity. Thus, cells can rarely be ideally matched for cellular experiments or, if available, only in small quantities. We reasoned that cryopreservation of human GCs may be helpful. Previous studies indicated feasibility of such an approach, but low survival of GCs was reported and consequences on GC functionality were only partially evaluated. We tested a slow freezing protocol (employing FCS and DMSO) of GCs upon isolation from follicular fluid. We compared cryopreserved and subsequently thawed cells with fresh, not cryopreserved ones, from the same patients. About 80 % of human GCs survived freezing/thawing. Neither morphology, nor levels of cell-cell contact, mitochondrial and steroidogenic genes were different between the two groups in cells cultured for 1-5 days. A proteomic analysis revealed no statistical significance in the abundance of a total of 5962 proteins. Both groups produced comparable basal levels of progesterone and responded similarly to hCG with elevation of progesterone. Taken together, we describe a rapid and readily available method for the cryopreservation of human GCs. We anticipate that it will allow future large-scale experiments and may thereby improve cellular studies with human ovarian cells.
HostingRepository	PRIDE
AnnounceDate	2021-07-29
AnnouncementXML	Submission_2021-07-28_22:21:36.766.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Ignasi Forne
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	timsTOF Pro

Revision	Datetime	Status	ChangeLog Entry
0	2021-04-26 01:20:11	ID requested
1	2021-07-28 01:01:18	announced
⏵ 2	2021-07-28 22:21:37	announced	2021-07-29: Updated project metadata.

Beschta S, Eubler K, Bohne N, Forne I, Berg D, Berg U, Mayerhofer A, A rapid and robust method for the cryopreservation of human granulosa cells. Histochem Cell Biol, 156(5):509-517(2021) [pubmed]

10.1007/S00418-021-02019-3;

submitter keyword: mass spectrometry, IVF, cell culture, progesterone, ovarian cells,cryopreservation

Prof. Dr. Artur Mayerhofer
contact affiliation	Department for Cell Biology BioMedizinisches Centrum - BMC LMU Muenchen, Med. Faculty Großhaderner Straße 9 82152 Planegg-Martinsried
contact email	mayerhofer@bmc.med.lmu.de
lab head
Ignasi Forne
contact affiliation	Biomedical Center-LMU
contact email	ignasi.forne@lrz.uni-muenchen.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/07/PXD025632

PRIDE project URI

• PRIDE
  1. PXD025632
     1. Label: PRIDE project
     2. Name: Analysis of cryopreservation of human granulosa cells