PXD025631

PXD025631 is an original dataset announced via ProteomeXchange.

Title	The acyl-proteome of Syntrophus aciditrophicus reveals metabolic relationship with benzoate degradation
Description	Syntrophus aciditrophicus is a model syntrophic bacterium that degrades fatty and aromatic acids into acetate, CO2, formate and H2 that are utilized by methanogens and other hydrogen-consuming microbes. The degradation of benzoate by S. aciditrophicus proceeds by a multi-step pathway that involves many reactive acyl-Coenzyme A species (RACS) as intermediates which can potentially result in Nε-acylation of lysine residues in proteins. Herein, we investigate post-translational modifications in the S. aciditrophicus proteome to identify and characterize a variety of acyl-lysine modifications that correspond to RACS present in the benzoate degradation pathway. Modification levels are sufficient to support post-translational modification analyses without antibody enrichment, enabling the study of a range of acylations located, presumably, on the most extensively acylated proteins. Seven types of acyl modifications were identified throughout the proteome, six of which correspond directly to RACS that are intermediates in the benzoate degradation pathway. Benzoate–degrading proteins are heavily represented among acylated proteins. The presence of functional deacylase enzymes in S. aciditrophicus indicates a potential regulatory system/mechanism by which these bacteria modulate acylation. Uniquely, Nε-acyl-lysine RACS are highly abundant in these syntrophic bacteria, raising the compelling possibility of enzyme modulation during benzoate degradation in this, and potentially, other syntrophic bacteria. Our results outline candidates to further study the impact of acylations within syntrophic systems.
HostingRepository	PRIDE
AnnounceDate	2022-03-16
AnnouncementXML	Submission_2022-03-16_04:08:51.008.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Janine Fu
SpeciesList	scientific name: Syntrophus aciditrophicus SB; NCBI TaxID: 56780;
ModificationList	monohydroxylated residue; acetylated residue; iodoacetamide derivatized residue
Instrument	QSTAR

Revision	Datetime	Status	ChangeLog Entry
0	2021-04-26 01:10:19	ID requested
⏵ 1	2022-03-16 04:08:51	announced

Muroski JM, Fu JY, Nguyen HH, Wofford NQ, Mouttaki H, James KL, McInerney MJ, Gunsalus RP, Loo JA, Ogorzalek Loo RR, The Acyl-Proteome of Syntrophus aciditrophicus Reveals Metabolic Relationships in Benzoate Degradation. Mol Cell Proteomics, 21(4):100215(2022) [pubmed]

submitter keyword: Lysine acetylation, lysine acylation, Syntrophs, LC-MS/MS, 2D-PAGE,

Joseph A. Loo
contact affiliation	Department of Chemistry and Biochemistry, University of California, Los Angeles, CA,USA David Geffen School of Medicine, Department of Biological Chemistry, University of California, Los Angeles, CA, USA UCLA-DOE Institute, University of California, Los Angeles, CA, USA UCLA Molecular Biology Institute, University of California, Los Angeles, CA, USA
contact email	jloo@chem.ucla.edu
lab head
Janine Fu
contact affiliation	University of California, Los Angeles
contact email	janinefu@chem.ucla.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD025631
     1. Label: PRIDE project
     2. Name: The acyl-proteome of Syntrophus aciditrophicus reveals metabolic relationship with benzoate degradation